Transcriptome combined with population level validation reveals genomic loci controlling plant height in flax (Linum usitatissimum L.)

Plant height is a key agronomic trait in bidirectional regulation of flax bast fiber and seeds yield. Although plant height is significantly distinct between oil flax and fiber flax, the genetic basis of plant height remains largely unknown. In this study, plant height related genes in model species Arabidopsis thaliana and rice (Oryza sativa L.) were homologous aligned to flax genome. And combined with the reported flax plant height candidate genes, 1593 genes were considered to have potential association with plant height in flax. Subsequently, a transcriptome analysis of tall and dwarf flax cultivars revealed that 532 candidates were differentially expression in young stem tissue above snap point, which were regarded as preliminary candidate genes for plant height. Next, the population level validation was performed using a core collection containing 200 flax accessions across four environments, and 30 genes repeatedly detected at least two environments were significantly associated with plant height. Taken together, our study provides a new strategy to rapid identify candidate genes associated with agronomic traits by combining transcriptome and population level validation. Meanwhile, our results provide valuable genomic resources and molecular insight into variation of plant height in flax subgroups, and may accelerate the improvement of high yield flax based on genomics-assisted breeding.

Automated summary

In this study, a total of 1593 genes were identified as potentially associated with plant height in flax through homologous alignment and transcriptome analysis. Through population level validation, 30 genes were consistently linked to plant height across different environments. These findings offer a new strategy to rapidly identify candidate genes related to agronomic traits and provide valuable genomic resources for improving high yield flax varieties through genomics-assisted breeding.