Journal
INDOOR AIR
Volume 32, Issue 1, Pages -Publisher
WILEY
DOI: 10.1111/ina.12945
Keywords
aerosol; DNA; nanoparticles; silica; tracing; ventilation
Categories
Funding
- ETH Zurich
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The use of silica particles with encapsulated DNA (SPED) as a tracing agent for measuring indoor aerosol distribution was successfully demonstrated in a series of experiments, showing the ability to quantitatively observe position dependency and ventilation effects in a confined space with detection limits below 0.1 ng particles per m(3) of sampled air. SPED show promise for a flexible, cost-effective, and low-impact characterization of aerosol dynamics in a wide range of settings.
Aerosolized particles play a significant role in human health and environmental risk management. The global importance of aerosol-related hazards, such as the circulation of pathogens and high levels of air pollutants, have led to a surging demand for suitable surrogate tracers to investigate the complex dynamics of airborne particles in real-world scenarios. In this study, we propose a novel approach using silica particles with encapsulated DNA (SPED) as a tracing agent for measuring aerosol distribution indoors. In a series of experiments with a portable setup, SPED were successfully aerosolized, recaptured, and quantified using quantitative polymerase chain reaction (qPCR). Position dependency and ventilation effects within a confined space could be shown in a quantitative fashion achieving detection limits below 0.1 ng particles per m(3) of sampled air. In conclusion, SPED show promise for a flexible, cost-effective, and low-impact characterization of aerosol dynamics in a wide range of settings.
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