Journal
IMMUNOLOGY LETTERS
Volume 241, Issue -, Pages 49-54Publisher
ELSEVIER
DOI: 10.1016/j.imlet.2021.12.002
Keywords
T-cell receptor; Prohibitin; p56 (lck) ; Vi polysaccharide
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Funding
- Department of Biotechnology, Government of India, through the National Institute of Immunology
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Prohibitin plays a regulatory role in Lck activation and TCR signaling, and its association with Lck and actin is involved in the secretion of IL-2 by T cells in response to Salmonella enterica serovar Typhi infection.
Prohibitin is a highly conserved ubiquitously expressed protein involved in several key cellular functions. Targeting of this protein in the membrane by the virulence polysaccharide, Vi, of human typhoid-causing pathogen, Salmonella enterica serovar Typhi (S. Typhi), results in suppression of IL-2 secretion from T cells activated through the T-cell receptor (TCR). However, the mechanism of this suppression remains unclear. Here, using Vi as a probe, we show that membrane prohibitin associates with the src-tyrosine kinase, p56(lck) (Lck), and actin in human model T cell line, Jurkat. Activation with anti-CD3 antibody brings about dissociation of this complex, which coincides with downstream ERK activation. The trimolecular complex reappears towards culmination of proximal TCR signaling. Engagement of cells with Vi prevents TCR-triggered activation of Lck and ERK by inhibiting dissociation of the former from prohibitin. These findings suggest a regulatory role for membrane prohibitin in Lck activation and TCR signaling.
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