4.6 Review

Imaging the different timescales of germinal center selection*

Related references

Note: Only part of the references are listed.
Article Immunology

Cyclin D3 drives inertial cell cycling in dark zone germinal center B cells

Juhee Pae et al.

Summary: During affinity maturation, GC B cells undergo proliferation and hypermutation in the dark zone, followed by selection in the light zone. Cyclin D3 plays a crucial role in controlling the anatomical segregation and cell cycle of GC B cells, and a specific mutation can lead to clonal B cell lymphoproliferation.

JOURNAL OF EXPERIMENTAL MEDICINE (2021)

Article Biology

Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages

Carolin Ulbricht et al.

Summary: Calcium concentration plays a crucial role in immune cell development and response to pathogens. With a novel numerical analysis, absolute cytoplasmic calcium concentrations in activated B cells have been successfully extracted for the first time.

ELIFE (2021)

Review Immunology

Heterogeneity of germinal center B cells: New insights from single-cell studies

Noudjoud Attaf et al.

Summary: Recent studies on GC B-cell heterogeneity using single-cell technologies have challenged the two-state DZ-LZ paradigm, revealing the cellular and molecular heterogeneity of GC B cells, including different cell states and potential cell fates.

EUROPEAN JOURNAL OF IMMUNOLOGY (2021)

Article Immunology

The immunodominant antibody response to Zika virus NS1 protein is characterized by cross-reactivity to self

Cecilia B. Cavazzoni et al.

Summary: The study found that Zika virus NS1 is an immunodominant viral antigen associated with the presence of autoreactive antibodies in humoral immune response. This suggests a potential role for NS1 in ZIKV-associated autoimmune manifestations.

JOURNAL OF EXPERIMENTAL MEDICINE (2021)

Article Immunology

Germinal center-dependent and -independent memory B cells produced throughout the immune response

Charlotte Viant et al.

Summary: This study utilized an unbiased lineage-tracking approach to investigate the origins and properties of memory B cell subsets in mice with intact immune system, revealing that activated B cells continue to differentiate into memory B cells throughout the immune response. Memory B cells originating from activated B cells or GCs display differences in isotype, overall gene expression, somatic hypermutation, and antigen affinity.

JOURNAL OF EXPERIMENTAL MEDICINE (2021)

Article Multidisciplinary Sciences

SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses

Jackson S. Turner et al.

Summary: SARS-CoV-2 mRNA vaccines induce a persistent germinal centre B cell response in humans, leading to the generation of robust humoral immunity, especially more significant in individuals previously infected with the virus.

NATURE (2021)

Article Biochemical Research Methods

High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy

Jeffrey Demas et al.

Summary: Two-photon microscopy has enabled high-resolution imaging of neuroactivity within scattering brain tissue, but there are still tradeoffs between speed and spatiotemporal sampling. Light beads microscopy (LBM) introduces a scalable and spatiotemporally optimal acquisition approach for recording neuroactivity at different scales, offering opportunities for discovering neurocomputations in the mammalian brain.

NATURE METHODS (2021)

Article Multidisciplinary Sciences

Expression of Foxp3 by T follicular helper cells in end-stage germinal centers

Johanne T. Jacobsen et al.

Summary: Research shows that contraction of immunization-induced germinal centers is associated with an acute surge in GC-resident Foxp3(+) T cells, attributed to the up-regulation of Foxp3 by T follicular helper cells. This natural up-regulation of Foxp3 in T-FH cells may play a role in regulating GC lifetimes.

SCIENCE (2021)

Review Immunology

Germinal Centre Shutdown

Theinmozhi Arulraj et al.

Summary: Germinal Centres (GCs) are structures in secondary lymphoid organs where B cell affinity maturation takes place following infection, but the natural termination of GC reaction lacks a clear mechanistic understanding.

FRONTIERS IN IMMUNOLOGY (2021)

Article Multidisciplinary Sciences

Longitudinal high-resolution imaging through a flexible intravital imaging window

Guillaume Jacquemin et al.

Summary: The study introduces a silicone-based imaging window for long-term, longitudinal intravital microscopy in mice. This low-cost device represents a significant technological advancement, allowing intravital imaging in diverse contexts in higher organisms and opening new avenues for in vivo imaging of soft and fragile tissues that were previously unattainable.

SCIENCE ADVANCES (2021)

Review Immunology

CRAC Channels and Calcium Signaling in T Cell-Mediated Immunity

Martin Vaeth et al.

TRENDS IN IMMUNOLOGY (2020)

Article Multidisciplinary Sciences

Tunable dynamics of B cell selection in gut germinal centres

Carla R. Nowosad et al.

NATURE (2020)

Article Immunology

Class-Switch Recombination Occurs Infrequently in Germinal Centers

Jonathan A. Roco et al.

IMMUNITY (2019)

Article Multidisciplinary Sciences

Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes

Imogen Moran et al.

NATURE COMMUNICATIONS (2018)

Article Biochemical Research Methods

Murine chronic lymph node window for longitudinal intravital lymph node imaging

Eelco F. J. Meijer et al.

NATURE PROTOCOLS (2017)

Article Multidisciplinary Sciences

The microanatomic segregation of selection by apoptosis in the germinal center

Christian T. Mayer et al.

SCIENCE (2017)

Article Biochemistry & Molecular Biology

Clonal Evolution of Autoreactive Germinal Centers

Soren E. Degn et al.

Review Immunology

Follicular Helper T Cells

Carola G. Vinuesa et al.

ANNUAL REVIEW OF IMMUNOLOGY, VOL 34 (2016)

Article Biochemistry & Molecular Biology

Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody

Mattia Bonsignori et al.

Review Immunology

Germinal Center B Cell Dynamics

Luka Mesin et al.

IMMUNITY (2016)

Article Immunology

Complex Antigens Drive Permissive Clonal Selection in Germinal Centers

Masayuki Kuraoka et al.

IMMUNITY (2016)

Article Multidisciplinary Sciences

Visualizing antibody affinity maturation in germinal centers

Jeroen M. J. Tas et al.

SCIENCE (2016)

Article Multidisciplinary Sciences

Loss of signalling via Gα13 in germinal centre B-cell-derived lymphoma

Jagan R. Muppidi et al.

NATURE (2014)

Article Multidisciplinary Sciences

Clonal selection in the germinal centre by regulated proliferation and hypermutation

Alexander D. Gitlin et al.

NATURE (2014)

Article Multidisciplinary Sciences

Dynamic signaling by T follicular helper cells during germinal center B cell selection

Ziv Shulman et al.

SCIENCE (2014)

Review Immunology

Follicular dendritic cells: dynamic antigen libraries

Balthasar A. Heesters et al.

NATURE REVIEWS IMMUNOLOGY (2014)

Article Biochemistry & Molecular Biology

The Proliferation-Quiescence Decision Is Controlled by a Bifurcation in CDK2 Activity at Mitotic Exit

Sabrina L. Spencer et al.

Article Immunology

Germinal center B cells govern their own fate via antibody feedback

Yang Zhang et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2013)

Article Biochemical Research Methods

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Carla P. Guimaraes et al.

NATURE PROTOCOLS (2013)

Article Multidisciplinary Sciences

T Follicular Helper Cell Dynamics in Germinal Centers

Ziv Shulman et al.

SCIENCE (2013)

Review Immunology

Germinal Centers

Gabriel D. Victora et al.

ANNUAL REVIEW OF IMMUNOLOGY, VOL 30 (2012)

Article Immunology

The proto-oncogene MYC is required for selection in the germinal center and cyclic reentry

David Dominguez-Sola et al.

NATURE IMMUNOLOGY (2012)

Review Immunology

Follicular Helper CD4 T Cells (T-FH)

Shane Crotty

ANNUAL REVIEW OF IMMUNOLOGY, VOL 29 (2011)

Article Immunology

A dynamic T cell-limited checkpoint regulates affinity-dependent B cell entry into the germinal center

Tanja A. Schwickert et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2011)

Article Multidisciplinary Sciences

Programming the magnitude and persistence of antibody responses with innate immunity

Sudhir Pai Kasturi et al.

NATURE (2011)

Article Multidisciplinary Sciences

Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding

Johannes F. Scheid et al.

SCIENCE (2011)

Article Immunology

B-cell fate decisions following influenza virus infection

Kristina Rothaeusler et al.

EUROPEAN JOURNAL OF IMMUNOLOGY (2010)

Article Immunology

Development and Migration of Plasma Cells in the Mouse Lymph Node

David R. Fooksman et al.

IMMUNITY (2010)

Article Immunology

Germinal center reutilization by newly activated B cells

Tanja A. Schwickert et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2009)

Article Immunology

Multiple layers of B cell memory with different effector functions

Ismail Dogan et al.

NATURE IMMUNOLOGY (2009)

Article Biochemical Research Methods

Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window

Anthony Holtmaat et al.

NATURE PROTOCOLS (2009)

Article Biochemistry & Molecular Biology

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression

Asako Sakaue-Sawano et al.

Review Immunology

Calcium signaling in lymphocytes

Masatsup Oh-Hora et al.

CURRENT OPINION IN IMMUNOLOGY (2008)

Article Medicine, Research & Experimental

Two-photon imaging of intratumoral CD8+ T cell cytotoxic activity during adoptive T cell therapy in mice

Beatrice Breart et al.

JOURNAL OF CLINICAL INVESTIGATION (2008)

Article Multidisciplinary Sciences

SAP-controlled T-B cell interactions underlie germinal centre formation

Hai Qi et al.

NATURE (2008)

Article Biochemical Research Methods

Intravital imaging of metastatic behavior through a mammary imaging window

Dmitriy Kedrin et al.

NATURE METHODS (2008)

Review Immunology

Germinal-center organization and cellular dynamics

Christopher D. C. Allen et al.

IMMUNITY (2007)

Article Immunology

In vivo imaging studies shed light on germinal-centre development

Anja E. Hauser et al.

NATURE REVIEWS IMMUNOLOGY (2007)

Article Multidisciplinary Sciences

In vivo imaging of germinal centres reveals a dynamic open structure

Tanja A. Schwickert et al.

NATURE (2007)

Article Multidisciplinary Sciences

Imaging of germinal center selection events during affinity maturation

Christopher D. C. Allen et al.

SCIENCE (2007)

Article Multidisciplinary Sciences

T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases

TR Mempel et al.

NATURE (2004)

Article Multidisciplinary Sciences

Two-photon imaging of lymphocyte motility and antigen response in intact lymph node

MJ Miller et al.

SCIENCE (2002)

Article Multidisciplinary Sciences

Dynamic imaging of T cell-dendritic cell interactions in lymph nodes

S Stoll et al.

SCIENCE (2002)

Article Biotechnology & Applied Microbiology

A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein

J Nakai et al.

NATURE BIOTECHNOLOGY (2001)