Journal
IEEE TRANSACTIONS ON NANOBIOSCIENCE
Volume 15, Issue 1, Pages 34-40Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2015.2508718
Keywords
Antiviral activity; foot-and-mouth disease virus; gold nanoparticle
Funding
- Shahid Chamran University of Ahvaz, Iran [92/4/04/874095]
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In this study, we evaluated the antiviral activity of gold nanoparticles (AuNPs) against the foot-and-mouth disease virus (FMDV), that causes a contagious disease in cloven-hoofed animals. The anti-FMDV activity of AuNPs was assessed using plaque reduction assay. MTT assay was used for quantitatively measuring the cytopathic effect caused by the viral infection. The 50% cytotoxicity concentration of nanoparticles was measured and found to be 10.4 mu g/ml. The virus yield reduction assay showed that AuNP have an approximately 4-fold virus titer reduction compared with controls. Plaque reduction assay showed that at non-cytotoxic concentrations, AuNPs do not show extracellular virucidal activity and inhibition of FMDV growth at the early stages of infection including attachment and penetration. Time-of-addition experiments revealed that AuNPs inhibited post-entry stages of viral replication concomitant with the onset of intracellular viral RNA synthesis; however, the mechanism of AuNPs against FMDV was unclear.
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