Millimeter-Wave Heating In Vitro: Local Microscale Temperature Measurements Correlated to Heat Shock Cellular Response

Objective: Cellular sensitivity to heat is highly variable depending on the cell line. The aim of this paper is to assess the cellular sensitivity of the A375 melanoma cell line to continuous (CW) millimeter-waves (MMW) induced heating at 58.4 GHz, between 37 degrees C and 47 degrees C to get a deeper insight into optimization of thermal treatment of superficial skin cancer. Methods: Phosphorylation of heat shock protein 27 (HSP27) was mapped within an area of about 30 mm2 to visualize the variation of heat-induced cellular stress as a function of the distance from the waveguide aperture (MMW radiation source). A multiphysics computational approach was then adopted to yield both electromagnetic and thermal field distributions as well as corresponding specific absorption rate (SAR) and temperature elevation. Induced temperature rise was experimentally measured using a micro-thermocouple (mu TC). Results: Coupling of the incident electromagnetic (EM) field with mu TC leads was first characterized, and optimal mu TC placing was identified. HSP27 phosphorylation was induced at temperatures >= 41 degrees C, and its level increases as a function of the thermal dose delivered, remaining mostly focused within 3mm(2). Conclusion: Phosphorylation of HSP27 represents a valuable marker of cellular stress of A375 melanoma cells under MMW exposure, providing both quantitative and spatial information about the distribution of the thermal stress. Significance: These results may contribute to the design of thermal treatments of superficial melanoma through MMW-induced heating in the hyperthermic temperature range.

Automated summary

This study assessed the cellular sensitivity of A375 melanoma cells to continuous millimeter-wave (MMW) induced heating and observed the cellular stress through the phosphorylation of heat shock protein 27 (HSP27). The results showed that phosphorylation of HSP27 is a valuable marker for cellular stress in A375 melanoma cells under MMW exposure, providing quantitative and spatial information about the distribution of thermal stress.