4.3 Article

Methylation patterns of the CDKN2B and CDKN2A genes in an indigenous population exposed to pesticides

Journal

HUMAN & EXPERIMENTAL TOXICOLOGY
Volume 41, Issue -, Pages -

Publisher

SAGE PUBLICATIONS LTD
DOI: 10.1177/09603271211063161

Keywords

DNA methylation; ethnicity; pesticides; CDKN2B gene; CDKN2A gene

Categories

Funding

  1. CONACyT [262284]
  2. Red Tematica de Toxicologia de Plaguicidas [294303]

Ask authors/readers for more resources

This study aimed to investigate the methylation profiles of the CDKN2B and CDKN2A genes in a genetically conserved population exposed to pesticides. The results showed that the farmer group had a higher methylation percentage of CDKN2B than the non-farmer group, while no differences in CDKN2A were observed between groups. These results suggest that pesticide exposure and exposure time could modify the methylation pattern of the CDKN2B gene in Huichol indigenous individuals.
The INK4-ARF locus includes the CDKN2B and CDKN2A genes and is functionally relevant in the regulation of both cell proliferation and senescence. Studies have reported modifications of DNA methylation in this locus by exposure to environmental contaminants including pesticides; however, until now, specific methylation profiles have not been reported in genetically conserved populations exposed to occupational pesticides. The aim of this study was to determine the methylation profiles of the CDKN2B and CDKN2A genes in a genetically conserved population exposed to pesticides. A cross-sectional and analytical study was carried out in 190 Huichol indigenous persons. Information related to pesticide exposure, diet and other variables were obtained through the use of a structured questionnaire. Blood and urine samples were collected for methylation test and dialkylphosphates (DAP) determination, respectively. DNA methylation was measured by the pyrosequencing of bisulfite-treated DNA and DAP concentrations by gas chromatography-tandem mass spectrometry (GC/MS). The most frequent metabolite in the population was dimethylthiophosphate. The farmer group presented a higher methylation percentage of CDKN2B than the non-farmer group, but no differences in CDKN2A were observed between groups. A positive correlation between methylation of CpG site 3 of CDKN2B and time working in the field was observed in the farmer group. An association between methylation percentage of CDKN2B and age was also observed in the non-farmer group. These results suggest that pesticide exposure and exposure time in Huichol indigenous individuals could modify the methylation pattern of the CDKN2B gene.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available