Journal
GENOMICS
Volume 113, Issue 6, Pages 3967-3977Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2021.09.020
Keywords
CircRNA; ACSL1; Bovine adipocytes; Unsaturated fatty acids; RNA-seq
Funding
- National Natural Science Foundation [31860631]
- Young Doctor Fund Project of Gansu Provincial Department of Education [2021QB-027]
- Special Funds for Discipline Construction of Gansu Agricultural University [GAU-XKJS-2018031]
- Scientific Research Start-Up Funds for Openly-Recruited Doctors [GSAU-RCZX201711]
- Basic Research Innovation Group Project of Gansu Province [17JR5RA137]
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"Through RNA-Seq analysis, circRNAs were found to regulate the expression of the ACSL1 gene and other genes related to UFA synthesis in bovine adipocytes, providing insights into the molecular mechanisms involved in circRNA-mediated regulation of UFA synthesis."
Long-chain acyl-CoA synthetase 1 (ACSL1) is a member of the acyl-CoA synthetase family that plays a vital role in lipid metabolism. We have previously shown that the ACSL1 gene regulates the composition of unsaturated fatty acids (UFAs) in bovine skeletal muscle, which in turn regulates the fatty acid synthesis and the generation of lipid droplets. Here, we used RNA-Seq to screen circRNAs that regulated the expression of ACSL1 gene and other UFA synthesis-related genes by RNA interference and noninterference in bovine adipocytes. The results of KEGG pathway analysis showed that the parental genes of differentially expressed (DE)-circRNAs were primarily enriched in the adipocytokine signaling pathway. The prediction results showed that novel_circ_0004855, novel_circ_0001507, novel_circ_0001731, novel_circ_0005276, novel_circ_0002060, novel_circ_0005405 and novel_circ_0004254 regulated UFA synthesis-related genes by interacting with the related miRNAs. These results could help expand our knowledge of the molecular mechanisms of circRNAs in the regulation of UFA synthesis in bovine adipocytes.
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