Journal
GENOMICS
Volume 114, Issue 1, Pages 45-60Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2021.11.016
Keywords
Plant peroxidases; Soybean; Drought stress; Expression analysis
Funding
- National Key Research and Devel-opment Program [2018YFD0201006]
- National Natural Science Foundation of China [31871646, 31571691]
- MOE Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT_17R55]
- Fundamental Research Funds for the Central Universities [KYT201801]
- Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP) Program
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This study provides a comprehensive report on the characterization of the GmPOD gene family in soybean, including gene number, distribution, classification, and evolutionary mechanisms. Differential expression of some GmPODs between drought-tolerant and drought-sensitive genotypes was observed, with GsPOD40 enhancing drought tolerance in soybean by regulating defense response pathways.
Class III peroxidases (PODs) are plant-specific glycoproteins, that play essential roles in various plant physiological processes and defence responses. To date, scarce information is available about the POD gene family in soybean. Hence, the present study is the first comprehensive report about the genome-wide characterization of GmPOD gene family in soybean (Glycine max L.). Here, we identified a total of 124 GmPOD genes in soybean, that are unevenly distributed across the genome. Phylogenetic analysis classified them into six distinct sub-groups (AF), with one soybean specific subgroup. Exon-intron and motif analysis suggested the existence of structural and functional diversity among the sub-groups. Duplication analysis identified 58 paralogous gene pairs; segmental duplication and positive/Darwinian selection were observed as the major factors involved in the evolution of GmPODs. Furthermore, RNA-seq analysis revealed that 23 out of a total 124 GmPODs showed differential expression between drought-tolerant and drought-sensitive genotypes under stress conditions; however, two of them (GmPOD40 and GmPOD42) revealed the maximum deregulation in all contrasting genotypes. Over expression (OE) lines of GsPOD40 showed considerably higher drought tolerance compared to wild type (WT) plants under stress treatment. Moreover, the OE lines showed enhanced photosynthesis and enzymatic antioxidant activities under drought stress, resulting in alleviation of ROS induced oxidative damage. Hence, the GsPOD40 enhanced drought tolerance in soybean by regulating the key physiological and biochemical pathways involved in the defence response. Lastly, the results of our study will greatly assist in further functional characterization of GsPODs in plant growth and stress tolerance in soybean.
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