Journal
GENE EXPRESSION PATTERNS
Volume 42, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.gep.2021.119215
Keywords
Galloway-Mowat syndrome; KEOPS complex; Expression and disease
Categories
Funding
- Else Kroner-Fresenius Foundation [2018_A78]
- German Ministry of Education and Research (GeNeRARe) [01GM1519A]
- German Research Foundation [CRC1080]
- International Graduate School in Molecular Medicine at Ulm University
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Galloway-Mowat syndrome (GAMOS) is a rare developmental disease characterized by congenital brain anomalies and renal abnormalities. Mutations in multiple genes, including those encoding members of the KEOPS complex, have been implicated in GAMOS. In this study, the conservation and expression of KEOPS complex members Osgep, Tp53rk, and Tprkb across different species, including Xenopus laevis, were investigated during early embryonic development. The findings suggest an important role for the KEOPS complex in vertebrate embryogenesis.
Galloway-Mowat syndrome (GAMOS) is a rare developmental disease. Patients suffer from congenital brain anomalies combined with renal abnormalities often resulting in an early-onset steroid-resistant nephrotic syndrome. The etiology of GAMOS has a heterogeneous genetic contribution. Mutations in more than 10 different genes have been reported in GAMOS patients. Among these are mutations in four genes encoding members of the human KEOPS (kinase, endopeptidase and other proteins of small size) complex, including OSGEP, TP53RK, TPRKB and LAGE3. Until now, these components have been functionally mainly investigated in bacteria, eukarya and archaea and in humans in the context of the discovery of its role in GAMOS, but the KEOPS complex members' expression and function during embryogenesis in vertebrates is still unknown. In this study, in silico analysis showed that both gene localization and the protein sequences of the three core KEOPS complex members Osgep, Tp53rk and Tprkb are highly conserved across different species including Xenopus laevis. In addition, we examined the spatio-temporal expression pattern of osgep, tp53rk and tprkb using RT-PCR and whole mount in situ hybridization approaches during early Xenopus development. We observed that all three genes were expressed during early embryogenesis and enriched in tissues and organs affected in GAMOS. More precisely, KEOPS complex genes are expressed in the pronephros, but also in neural tissue such as the developing brain, eye and cranial cartilage. These findings suggest that the KEOPS complex plays an important role during vertebrate embryonic development.
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