What physicochemical properties of biochar facilitate interspecies electron transfer in anaerobic digestion: A case study of digestion of whiskey by-products

The efficiency of microbial interspecies electron transfer between syntrophic bacteria and methanogens is considered a rate-limiting factor for the overall efficiency of anaerobic digestion (AD). Stimulating interspecies electron transfer by biochars has been demonstrated to be efficient to enhance AD. However, the enhancing effects vary significantly depending on biochar properties. The correlations between them are not fully under-stood. Herein, biochars with different physicochemical properties were produced from a whiskey by-product draff and subsequently applied in the digestion of draff. The biochar produced at 700 degrees C statistically (p less than 0.05) enhanced biomethane yield by 5%. In contrast, biochars produced at 500 and 900 degrees C did not increase biomethane yield. The addition of 700 degrees C-derived biochar in AD increased the relative abundance of the methanogen Methanosarcina, which may be the electron-accepting partner in direct interspecies electron transfer (DIET). The enrichment of Methanosarcina suggested the potential shift of the interspecies electron transfer pathway towards the DIET mode. The characterization of biochar properties suggested that moderate graphiti-zation degree and abundant active surface functional groups (such as -C = O, pyridinic-N, and graphitic-N) were correlated with a more stimulating interspecies electron transfer through both the carbon matrices and the charging - discharging cycles of surface functional groups.

Automated summary

This study investigated the effects of biochar properties on enhancing interspecies electron transfer in anaerobic digestion (AD). Results showed that biochar produced at 700 degrees C significantly increased biomethane yield, while biochars produced at 500 and 900 degrees C did not show the same effect. The addition of biochar at 700 degrees C enriched the methanogen Methanosarcina, suggesting a shift towards direct interspecies electron transfer (DIET) pathway. Moderate graphitization degree and abundant active surface functional groups were found to be correlated with enhanced interspecies electron transfer through carbon matrices and surface functional groups.