Prooxidative inhibition against NF-κB-mediated inflammation by pharmacological vitamin C

Vitamin C (VC), widely found in vegetables and fruits, operates as an electron donor to perform various biological functions including anti-inflammatory activity. However, the mechanisms by which VC inhibits inflammation remain insufficiently understood. Accordingly, we performed a detail mechanistic study on antiinflammatory activity of VC at millimolar (pharmacological) concentrations in lipopolysaccharides-stimulated RAW264.7 cells. It was found that VC and its two-electron oxidative product, dehydroascorbate (DHA) constructs an efficient redox cycle with the aid of intracellular glutathione and copper ions, thereby facilitating the generation of reactive oxygen species (ROS) and the ROS-dependent inhibition against the NF-kappa B-mediated inflammation.

Automated summary

The study found that vitamin C can form an efficient redox cycle with intracellular glutathione and copper ions, generating reactive oxygen species (ROS), which in turn inhibits NF-kappa B-mediated inflammation.