4.7 Article

Thioredoxin reductase 1 inhibitor shikonin promotes cell necroptosis via SecTRAPs generation and oxygen-coupled redox cycling

Related references

Note: Only part of the references are listed.
Article Medicine, Research & Experimental

Plumbagin reduction by thioredoxin reductase 1 possesses synergy effects with GLUT1 inhibitor on KEAP1-mutant NSCLC cells

Shibo Sun et al.

Summary: Thioredoxin reductase 1 plays a crucial role in catalyzing the reduction of plumbagin and converting the enzyme from an antioxidant to a pro-oxidant. Plumbagin is ineffective against KEAP1-mutant non-small cell lung cancer cells, but its resistance can be overcome by inhibiting glucose transporter 1 (GLUT1) or glucose-6-phosphate dehydrogenase (G6PD).

BIOMEDICINE & PHARMACOTHERAPY (2022)

Review Chemistry, Medicinal

Small molecules regulating reactive oxygen species homeostasis for cancer therapy

Junmin Zhang et al.

Summary: Regulating intracellular ROS levels may be an effective strategy for cancer prevention or treatment. This can be achieved through chemoprevention to reduce ROS production pathways, or through ROS-mediated anticancer therapy to stimulate ROS clearance, ultimately inhibiting tumor growth.

MEDICINAL RESEARCH REVIEWS (2021)

Article Biochemistry & Molecular Biology

Natural Molecules Targeting Thioredoxin System and Their Therapeutic Potential

Junmin Zhang et al.

Summary: The Thioredoxin (Trx) system plays a vital role in maintaining cellular redox homeostasis and regulating multiple cellular redox signaling pathways, and has been shown to be an important regulator in many diseases, especially tumorigenesis. Developing potential therapeutic molecules targeting this system is crucial for disease treatment.

ANTIOXIDANTS & REDOX SIGNALING (2021)

Article Medicine, Research & Experimental

Functionalized PDA/DEX-PEI@HA nanoparticles combined with sleeping-beauty transposons for multistage targeted delivery of CRISPR/Cas9 gene

Kun Ma et al.

Summary: The CRISPR/Cas9 gene was cloned into a non-viral safe vector Sleeping-Beauty (SB) transposon to construct a safer vector. In addition, PDA/DEX-PEI@HA (PDPH) nanoparticles were developed to facilitate precise CRISPR/Cas9 targeted delivery, showing high transfection efficiency and minimal cytotoxicity. The study highlights the potential of PDA/DEX-PEI25k@HA/pT25pCas9 (PDPH25k/pT(25K)pCas9) as a non-viral gene vector for CRISPR/Cas9 delivery in clinical medication.

BIOMEDICINE & PHARMACOTHERAPY (2021)

Article Biochemistry & Molecular Biology

Synthesis and biological evaluation of disulfides as anticancer agents with thioredoxin inhibition

Xiangxu Wei et al.

Summary: The study synthesized 72 disulfides and identified two compounds with significant antitumor activity against HeLa cells. Kinetic studies showed one of the disulfides had faster inhibition of Trx compared to the other. These findings suggest potential for the development of anticancer candidates with Trx inhibition.

BIOORGANIC CHEMISTRY (2021)

Article Biochemistry & Molecular Biology

Efficient purification of selenoprotein thioredoxin reductase 1 by using chelating reagents to protect the affinity resins and rescue the enzyme activities

Shibo Sun et al.

Summary: Chelating reagents, such as 20 mM EDTA or EGTA, were found to effectively protect or rescue TrxR1 from heavy metal ions during affinity chromatography, leading to a significant increase in yield to over 80% and improving the reproducibility of the 2',5'-ADP Sepharose column. Additionally, the purified enzyme showed a single band on SDS-PAGE gel and exhibited a high specific activity of >20 IJ/mg, indicating successful purification and functionality.

PROCESS BIOCHEMISTRY (2021)

Article Biochemistry & Molecular Biology

Oxidative stress-mediated AMPK inactivation determines the high susceptibility of LKB1-mutant NSCLC cells to glucose starvation

Yi Ren et al.

Summary: Mutations in LKB1 lead to increased susceptibility of NSCLC cells to glucose starvation-induced cell death, which triggers oxidative stress and AMPK protein oxidation, eventually resulting in cell death. This process can be effectively reversed and rescued by 2DG, A769662, and NAC.

FREE RADICAL BIOLOGY AND MEDICINE (2021)

Article Multidisciplinary Sciences

Activation of the NRF2 antioxidant program sensitizes tumors to G6PD inhibition

Hongyu Ding et al.

Summary: The KEAP1/NRF2 pathway promotes metabolic rewiring to support redox homeostasis, and G6PD inhibition-induced TCA depletion is a therapeutic vulnerability of NRF2-activated cancer.

SCIENCE ADVANCES (2021)

Article Biochemistry & Molecular Biology

Chlorophyllin Inhibits Mammalian Thioredoxin Reductase 1 and Triggers Cancer Cell Death

Shibo Sun et al.

Summary: This study found that five commercial food colorants inhibited TrxR1 protein, with chlorophyll and betanin also triggering oligomerization of TrxR1. Furthermore, chlorophyllin irreversibly inhibited the reducing activity of TrxR1 and reduced cellular TrxR activity, leading to cancer cell death.

ANTIOXIDANTS (2021)

Article Biochemistry & Molecular Biology

Menadione inhibits thioredoxin reductase 1 via arylation at the Sec498 residue and enhances both NADPH oxidation and superoxide production in Sec498 to Cys498 substitution

Shibo Sun et al.

Summary: This study reveals that TrxR1 catalyzes the reduction of menadione in a Sec-independent manner, depending highly on Cys(498) instead. Mutation results showed that the U498C variant supported high-efficiency menadione reduction. The study also reveals that the Sec(498) of TrxR1 is the primary target of menadione.

FREE RADICAL BIOLOGY AND MEDICINE (2021)

Review Pharmacology & Pharmacy

Thioredoxin reductase as a pharmacological target

Geir Bjorklund et al.

Summary: Thioredoxin reductases (TrxRs) are enzymes that reduce thioredoxin, playing a crucial role in the antioxidant system within cells. Inhibition of TrxR is a key clinical goal to address redox balance disturbances. Current research focuses on the importance of TrxRs in cellular functions and the mechanisms of inhibition by metal ions or chemicals.

PHARMACOLOGICAL RESEARCH (2021)

Article Multidisciplinary Sciences

KEAP1 deficiency drives glucose dependency and sensitizes lung cancer cells and tumors to GLUT inhibition

Pranavi Koppula et al.

Summary: KEAP1 deficiency in lung cancer cells increases glucose dependency, making them more vulnerable to glucose deprivation. This study reveals a potential therapeutic target by targeting metabolic vulnerabilities in KEAP1-mutant lung cancer.

ISCIENCE (2021)

Article Biochemistry & Molecular Biology

Inhibition of TXNRD or SOD1 overcomes NRF2-mediated resistance to β-lapachone

Laura Torrente et al.

REDOX BIOLOGY (2020)

Review Oncology

Oxidative Stress in Cancer

John D. Hayes et al.

CANCER CELL (2020)

Article Multidisciplinary Sciences

GLUT1 inhibition blocks growth of RB1-positive triple negative breast cancer

Qin Wu et al.

NATURE COMMUNICATIONS (2020)

Article Oncology

Keap1 mutation renders lung adenocarcinomas dependent on Slc33a1

Rodrigo Romero et al.

NATURE CANCER (2020)

Article Biochemistry & Molecular Biology

Shikonin induces colorectal carcinoma cells apoptosis and autophagy by targeting galectin-1/JNK signaling axis

Nan Zhang et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES (2020)

Review Chemistry, Medicinal

Small molecule inhibitors of mammalian thioredoxin reductase as potential anticancer agents: An update

Junmin Zhang et al.

MEDICINAL RESEARCH REVIEWS (2019)

Article Oncology

Shikonin derivatives for cancer prevention and therapy

Joelle C. Boulos et al.

CANCER LETTERS (2019)

Article Biochemistry & Molecular Biology

Promotion of HeLa cells apoptosis by cynaropicrin involving inhibition of thioredoxin reductase and induction of oxidative stress

Tianyu Liu et al.

FREE RADICAL BIOLOGY AND MEDICINE (2019)

Article Biochemistry & Molecular Biology

The combination of ascorbate and menadione causes cancer cell death by oxidative stress and replicative stress

Xiaoyuan Ren et al.

FREE RADICAL BIOLOGY AND MEDICINE (2019)

Review Pharmacology & Pharmacy

Pharmacological properties and derivatives of shikonin-A review in recent years

Chuanjie Guo et al.

PHARMACOLOGICAL RESEARCH (2019)

Review Oncology

NRF2 and the Hallmarks of Cancer

Montserrat Rojo de la Vega et al.

CANCER CELL (2018)

Article Multidisciplinary Sciences

Shikonin Inhibits Tumor Growth in Mice by Suppressing Pyruvate Kinase M2-mediated Aerobic Glycolysis

Xiaoyue Zhao et al.

SCIENTIFIC REPORTS (2018)

Review Pharmacology & Pharmacy

Targeting the Thioredoxin System for Cancer Therapy

Junmin Zhang et al.

TRENDS IN PHARMACOLOGICAL SCIENCES (2017)

Article Pharmacology & Pharmacy

Identification of new shikonin derivatives as STAT3 inhibitors

Han-Yue Qiu et al.

BIOCHEMICAL PHARMACOLOGY (2017)

Article Biochemistry & Molecular Biology

Selenium as an Electron Acceptor during the Catalytic Mechanism of Thioredoxin Reductase

Adam P. Lothrop et al.

BIOCHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Why Is Mammalian Thioredoxin Reductase 1 So Dependent upon the Use of Selenium?

Adam P. Lothrop et al.

BIOCHEMISTRY (2014)

Review Biochemistry & Molecular Biology

The thioredoxin antioxidant system

Jun Lu et al.

FREE RADICAL BIOLOGY AND MEDICINE (2014)

Article Biochemistry & Molecular Biology

Shikonin targets cytosolic thioredoxin reductase to induce ROS-mediated apoptosis in human promyelocytic leukemia HL-60 cells

Dongzhu Duan et al.

FREE RADICAL BIOLOGY AND MEDICINE (2014)

Review Biochemistry & Molecular Biology

Selenium and selenocysteine: roles in cancer, health, and development

Dolph L. Hatfield et al.

TRENDS IN BIOCHEMICAL SCIENCES (2014)

Article Biochemistry & Molecular Biology

Wobble decoding by the Escherichia coli selenocysteine insertion machinery

Jianqiang Xu et al.

NUCLEIC ACIDS RESEARCH (2013)

Review Pharmacology & Pharmacy

The Chemical Biology of Naphthoquinones and Its Environmental Implications

Yoshito Kumagai et al.

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, VOL 52 (2012)

Article Biochemistry & Molecular Biology

In vivo redox state of Human thioredoxin and redox shift by the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA)

J. Ungerstedt et al.

FREE RADICAL BIOLOGY AND MEDICINE (2012)

Article Multidisciplinary Sciences

Crystal structure of the human thioredoxin reductase-thioredoxin complex

Karin Fritz-Wolf et al.

NATURE COMMUNICATIONS (2011)

Review Biochemistry & Molecular Biology

Focus on mammalian thioredoxin reductases - Important selenoproteins with versatile functions

Elias S. J. Arner

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2009)

Article Biochemistry & Molecular Biology

High levels of thioredoxin reductase 1 modulate drug-specific cytotoxic efficacy

Sofl E. Eriksson et al.

FREE RADICAL BIOLOGY AND MEDICINE (2009)

Article Biochemistry & Molecular Biology

Crystal Structure and Catalysis of the Selenoprotein Thioredoxin Reductase 1

Qing Cheng et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Biochemistry & Molecular Biology

Molecular Mechanisms of Thioredoxin and Glutaredoxin as Hydrogen Donors for Mammalian S Phase Ribonucleotide Reductase

Farnaz Zahedi Avval et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Review Biotechnology & Applied Microbiology

Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach?

Dunyaporn Trachootham et al.

NATURE REVIEWS DRUG DISCOVERY (2009)

Article Biochemistry & Molecular Biology

Inhibition of the human thioredoxin system - A molecular mechanism of mercury toxicity

Cristina M. L. Carvalho et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2008)

Article Multidisciplinary Sciences

Cell Death by SecTRAPs: Thioredoxin Reductase as a Prooxidant Killer of Cells

Karin Anestal et al.

PLOS ONE (2008)

Article Biochemistry & Molecular Biology

The structure of human thioredoxin reductase 1 provides insights into C-terminal rearrangements during catalysis

Karin Fritz-Wolf et al.

JOURNAL OF MOLECULAR BIOLOGY (2007)

Article Multidisciplinary Sciences

Crystal structures of oxidized and reduced mitochondrial thioredoxin reductase provide molecular details of the reaction mechanism

EI Biterova et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)

Article Biochemistry & Molecular Biology

Interactions of quinones with thioredoxin reductase -: A challenge to the antioxidant role of the mammalian selenoprotein

N Cenas et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2004)

Article Multidisciplinary Sciences

Three-dimensional structure of a mammalian thioredoxin reductase: Implications for mechanism and evolution of a selenocysteine-dependent enzyme

T Sandalova et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)

Article Multidisciplinary Sciences

Structure and mechanism of mammalian thioredoxin reductase:: The active site is a redox-active selenolthiol/selenenylsulfide formed from the conserved cysteine-selenocysteine sequence

LW Zhong et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)