Exploring a degradation strategy for biogenic amines based on the Cantonese soy sauce production method

Biogenic amines (BAs) and ethyl carbamate (EC) are of great concern to society as potential endogenous hazards produced during traditional fermented food processing. In this work, whether the change in microbial community diversity in Cantonese soy sauce resulted in the differences in BA and EC contents was investigated. BAs, especially phenethylamine, tyramine, and histamine, were positively correlated with Lactobacillus and Weissella, while EC was positively related to Staphylococcus. The results indicated that BA and EC share metabolic pathways, in which the putrescine precursor ornithine is converted into citrulline and then reacts with ethanol to form EC. The ability of Bacillus isolated from the moromi to produce BA was strongest during the middle phase of the process. The contents of BAs involved in histamine were reduced by 66.42% and 69.31% by inoculating Zygosaccharomyces rouxii QH-17 and Tetragenococcus halophilus CGMCC3792, respectively. The contents of BAs were significantly reduced by coculturing T. halophilus CGMCC3792 with Z. rouxii QH-17 once the moromi was polluted during the koji manufacturing process or the initial phase of moromi preparation. The results show that one of the effective strategies for decreasing BAs in the soy sauce process is a biodisturbance strategy based on the functional microbiota.

Automated summary

The study revealed that Bacillus isolated from the moromi had the strongest ability to produce BA during the middle phase of the process. The contents of BAs involved in histamine were significantly reduced by inoculating Zygosaccharomyces rouxii QH-17 and Tetragenococcus halophilus CGMCC3792. A biodisturbance strategy based on the functional microbiota was identified as an effective approach to decrease BAs in the soy sauce process.