Journal
FOOD CHEMISTRY
Volume 367, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130754
Keywords
Neu5Ac; FRET; Exonuclease III; Recycling amplification; Fluorescent biosensor
Funding
- Six Talent Peaks Project in Jiangsu Province [JY-078]
- Postgraduate Research & Practice Innovation Program of Jiangnan University [JNKY19_011]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20_1826]
Ask authors/readers for more resources
A high-sensitive fluorescent biosensor for Neu5Ac has been developed based on FRET between CdSe/ZnS quantum dots (QDs) and BHQ(2), as well as exonuclease III (Exo III)-assisted recycling amplification strategy. The biosensor achieved an ultralow detection limit of 24 fM and good linear response ranges within 0.2-12.5 pM and 12.5-1000 pM, showing great potential for detection of sialic acids modified glycans/lipids in medical diagnosis and food testing.
N-acetylneuraminic acid (Neu5Ac) is widely spread in many biologically significant glycans of mammals, commonly as a terminal alpha-glycoside. It is of great significance to develop analytical techniques for detection of Neu5Ac. Herein, a high-sensitive fluorescent biosensor for Neu5Ac has been developed based on FRET between CdSe/ZnS quantum dots (QDs) and BHQ(2), as well as exonuclease III (Exo III)-assisted recycling amplification strategy. Employing the specially designed three-level FRET systems and fluorescent signal recovery mechanism, together with five-step recycling signal amplification chain reactions, an ultralow detection limit of 24 fM was achieved. Meanwhile, good linear response ranges within 0.2-12.5 pM and 12.5-1000 pM were founded. The assay has excellent performance in real sample detection, and thus offers great potential for detection of sialic acids modified glycans/lipids in the fields of medical diagnosis and food testing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available