Thermal degradation of stigmasterol under the deodorisation temperature exposure alone and in edible corn oil

Phytosterols are commonly found in vegetable oils and possess health benefits for humans. While investigating the chemical conversion of stigmasterol at deodorisation temperatures, gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) experiments led to the identification of 5-ethyl-6-methyl-3-heptene-2-one, 3-hydoxy-steroid, 3ketostigmasterol, and 3,7-diketostigmasterol as by-products. The identification of these compounds assisted in the interpretation of the stigmasterol oligomers characterised by high-pressure size exclusion chromatography (HPSEC). A similar analysis was conducted in stripped corn oil at the deodorisation temperatures. As such, 5ethyl-6-methyl-3-heptene-2-one, 3-hydoxy-steroid, 3-ketostigmasterol and 3,7-diketostigmasterol were also detected in stripped corn oil, while the contents of 3-hydoxy-steroid and 5-ethyl-6-methyl-3-heptene-2-one were higher than those of 3-ketostigmasterol, as revealed by quantum chemical simulations. In addition, stripped corn oil exhibited the characteristic of preventing stigmasterol degradation below 200 degrees C, whereas it enhanced the chemical conversion (such as esterification and degradation) of stigmasterol at higher temperatures.

Automated summary

Phytosterols found in vegetable oils have health benefits for humans. Chemical conversion of stigmasterol at deodorisation temperatures led to the identification of various by-products using GC-MS and UHPLC-HRMS experiments, helping to interpret stigmasterol oligomers. Similar analysis in stripped corn oil showed similar results, indicating different effects on stigmasterol at different temperatures.