Journal
FOOD CHEMISTRY
Volume 364, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130396
Keywords
Prussian blue; Histamine; Flow injection system; Gold nanoparticles; Cryogel
Funding
- Prince of Songkla University [SCI610483d]
- Faculty of Science for Natha Nontipichet (Research Assistantship) [2-2561-02-016]
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A histamine biosensor was developed using a Prussian blue-modified carbon electrode for detecting histamine levels in fish and shrimps. The biosensor demonstrated good specificity, high operational stability, and excellent long-term stability. It successfully measured different levels of histamine through two linear ranges.
A histamine biosensor was developed based on a screen-printed carbon electrode modified with Prussian blue (PB) electrodeposited on multi-walled carbon nanotubes covered with a macroporous layer of chitosan-gold nanoparticles composite cryogel (CS-AuNPs Cry). With its high specific surface area and conductivity, CS-AuNPs Cry proved an excellent supporting material for diamine oxidase (DAO) immobilization. PB acted as a redox mediator to promote electron transfer between hydrogen peroxide and the electrode surface. The PB reduction current was measured during the hydrogen peroxide-releasing oxidation of histamine catalyzed by DAO. The proposed biosensor displayed two linear ranges: 2.50-125.0 mu mol L-1 and 125.0-400.0 mu mol L-1. The limit of detection was 1.81 mu mol L-1. Reproducibility was good (RSD = 5.46%), operational stability high, long-term stability excellent, and selectivity good. The biosensor determined histamine levels in fish and shrimps with satisfactory recoveries, and the obtained results agreed with those obtained by ELISA.
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