Journal
FOOD CHEMISTRY
Volume 366, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130648
Keywords
Chloramphenicol; DNA four-arm junctions; Fluorescence; Aptasensor; Label-free
Funding
- National Natural Science Foundation of China [32001802]
- Large Instrument Open Foundation of Shandong Normal University [KFJJ2021004]
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A novel label-free fluorescence aptasensor was developed for chloramphenicol (CAP) detection by DNA fourarm junction-assisted target recycling and SYBR Green I dye-aided fluorescence-signal amplification. The method showed high sensitivity and specificity, making it suitable for chloramphenicol detection in food products.
A novel label-free fluorescence aptasensor was established for chloramphenicol (CAP) detection by DNA fourarm junction-assisted target recycling and SYBR Green I dye-aided fluorescence-signal amplification. The CAP aptamer was hybridized to its complementary strand (primer) to form a double-stranded primer/aptamer complex. In the presence of CAP, aptamers can specifically bind with CAP to dissociate primers, which can trigger the self-assembly of four hairpins to continuously generate DNA four-arm junctions. After digesting the excess hairpins using T7 exonuclease, SYBR Green I was inserted into the base pair-rich DNA four-arm junctions, which led to a significant increase in fluorescence intensity. Under optimal conditions, the developed aptasensor can detect CAP in a linear range of 1.0 pg mL-1 to 10 ng mL-1 with a detection limit of 0.72 pg mL-1. The recovery rates in milk and honey ranged from 90.3% to 106.6%. Thus, the method shows substantial potential for CAP detection in food products.
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