Journal
FOOD CHEMISTRY
Volume 364, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130362
Keywords
Whole wheat bread; Consumer liking; Untargeted chemical profiling; Trihydroxy fatty acid; Phosphocholine
Funding
- USDA National Institute of Food and Agriculture [20186701727557]
- USDA National Institute of Food and Agriculture, Hatch project [OHO101231]
- Flavor Research and Education Center at The Ohio State University
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Untargeted LC-MS flavoromic analysis was used to identify chemical compounds affecting consumer liking of whole wheat bread. The study found that specific compounds, including trihydroxy fatty acids, significantly contributed to the perceived bitterness intensity and overall negative impact on flavor liking of the bread samples. Lipoxygenase activity of the flour was reported to play a key role in the formation of these bitter compounds.
Untargeted LC-MS flavoromic analysis was utilized to identify chemical compounds that impact consumer liking of whole wheat bread. Chemical fingerprints of thirteen whole wheat breads were modeled against consumer flavor liking scores by orthogonal partial least squares (OPLS) with good fit ((RY)-Y-2 = 0.98) and predictive ability (Q(2) = 0.95). The four most predictive features (negatively correlated) were identified as 9S,12S,13S-trihydroxyoctadec-10E-enoic acid (pinellic acid), 9S,12S,13S-trihydroxy-octadeca-10E,15Z-dienoic acid, 8R*,9R*,10S*trihydroxy-octadec-6Z-enoic acid, and 1-(octadeca-9Z,12Z-dienoyl)-sn-glycero-3-phosphocholine. Sensory validation studies including bitter threshold determination and recombination tests confirmed the contribution of these compounds to the perceived bitterness intensity of the bread samples and the overall negative impact on flavor liking. Lipoxygenase activity of the flour was reported to have a significant impact on the formation of the three bitter compounds (trihydroxy fatty acids) in the bread samples.
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