Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 160, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2021.112769
Keywords
Black cohosh; Botanical dietary supplements; Phytochemical composition; Constituents
Categories
Funding
- Intramural Research Program of the NIH, National Institute of Environmental Health Sciences, Intramural Research project [ZIC ES103316-05]
- U.S. Department of Health and Human Services [HHSN273201400027C]
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The selection of a suitable black cohosh root extract for NTP evaluation is crucial, requiring confirmation of authenticity and purity. Through chemical analysis, the extract has been found to contain the desired constituents, ensuring its suitability for use.
Black cohosh (Actaea racemosa L.) is a botanical supplement marketed to women of all ages. Due to paucity of data to assess the safe use, the National Toxicology Program (NTP) is evaluating the toxicity of black cohosh. The use of an authentic, quality material is imperative to generate robust data. Because botanical materials are complex mixtures with variable composition, the selection of a material is challenging. We describe selection and phytochemical characterization of an unformulated black cohosh root extract (i.e., an extract that serves as source material for a formulated product) to be used in the NTP assessments. A material was selected using a combination of non-targeted and targeted chemical analyses, including confirmation of authenticity, absence of contaminants and adulterants, and similarity to a popular black cohosh product used by consumers. Thirty-nine constituents covering three major classes, triterpene glycosides, phenolic acids, and alkaloids were identified. Among constituents quantified, triterpene glycosides made up approximately 4.7% (w/w) with total constituents quantified making up 5.8% (w/w) of the extract. Non-targeted chemical analysis followed by chemometric analysis of various materials sold as black cohosh, and reference materials for black cohosh and other Actaea species further confirmed the suitability of the selected extract for use.
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