4.7 Review

Conventional and Emerging Techniques for Detection of Foodborne Pathogens in Horticulture Crops: a Leap to Food Safety

Journal

FOOD AND BIOPROCESS TECHNOLOGY
Volume 15, Issue 6, Pages 1248-1267

Publisher

SPRINGER
DOI: 10.1007/s11947-021-02730-y

Keywords

Fruits and vegetables; Flow cytometry; Biosensors; PCR; Immunological methods

Funding

  1. National Key Research and Development Program of China [2019YFC1604504]
  2. Scientific Research Fund of Zhejiang Provincial Education Department [Y202045626]

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Foodborne pathogen contamination in horticulture crops is a serious concern to human health. Conventional detection techniques have limitations, while recent advancement techniques offer advantages. This review summarizes various methods for detecting foodborne pathogens in horticulture crops and discusses the application of nanomaterials in biosensors and the combination of multiple methods.
Foodborne pathogen contamination in horticulture crops has become a serious concern to human health. Several methods for detecting foodborne pathogens in horticulture crops were employed to manage and anticipate these pathogens. Convectional techniques for foodborne pathogens detection have some disadvantages, including time-consuming, low sensitivity, and labor-intensive. The outstanding properties of recent advancement techniques possess the advantages of rapid, sensitive, effective, and time-saving. The current review comprehensively recaps the advanced of conventional and emerging trends in horticulture crops, such as culture-based methods, microscopic based methods, immunological based methods, molecular-based methods, and biosensors devices in horticulture crops and their influence on nanomaterials such as AuNPs or MWCNTs in the biosensors and a combination of more than one method such as ELISA with duplex PCR. Also, the recent advances and future prospects of these detection methods were covered.

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