Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Volume 95, Issue 6, Pages 745-751Publisher
SPRINGER
DOI: 10.1007/s00128-015-1626-1
Keywords
Hatching test; Acute and long-term mortality test; Artemia; Lethal and developmental/behavioral endpoint; Sensitivity rank of bioassays
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Funding
- Tor Vergata University/Regione Lazio Research Program
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Artemia sp. is extensively used in ecotoxicity testing, despite criticisms inherent to both acute and long-term tests. Alternative endpoints and procedures should be considered to support the use of this biological model. The hatching process comprises several developmental steps and the cyst hatchability seems acceptable as endpoint criterion. In this study, we assessed the reliability of the hatching assay on A. franciscana by comparing with acute and long-term mortality tests, using two chemicals: Diethylene Glycol (DEG), Sodium Dodecyl Sulphate (SDS). Both DEG and SDS tests demonstrated a dose dependent hatching inhibition. The hatching test resulted more sensitive than acute mortality test and less sensitive than the long-term one. Results demonstrate the reliability and high sensitivity of this hatching assay on a short time lag and support its useful application in first-tier risk assessment procedures.
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