Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Volume 95, Issue 6, Pages 758-763Publisher
SPRINGER
DOI: 10.1007/s00128-015-1577-6
Keywords
Chemcatcher passive sampler; Yeast-based two-hybrid recombinant receptor-reporter gene assay activity; Estrogenic activity; Aryl hydrocarbon receptor activity; Yarra River; Australia
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Funding
- Department of Primary Industries Victoria [08160, 06889]
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This pilot study was initiated to provide new information on the 'hormonal' activity of Victorian rivers. Chemcatcher (TM) passive sampler systems containing Empore (TM) C18FF disks were deployed at eight riverine sites near Melbourne. Little estrogenic activity [< 0.4-1.8 ng estradiol equivalents (EQ)/disk] and no retinoic acid activity (RAR, all samples < 0.8 ng trans-retinoic acid EQ/disk) was observed. Almost all sample extracts showed aryl hydrocarbon receptor activity (from < 4 to 29 ng beta-naphthoflavone EQ/disk). Overall, the disk extracts were eminently compatible with the bioassay screening technology, enabling the relative levels of 'hormonal activity' to be observed in the surface waters in and around Melbourne. From a practical perspective, the in situ sampling and pre-concentration provided by passive sampling reduces the manual handling risks associated with sample transport, and the number of laboratory operations required to obtain assay-ready solutions for analysis.
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