Journal
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
Volume 20, Issue 4, Pages 621-629Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14787210.2022.1990042
Keywords
Anthelminthics; drug discovery; drug repurposing; Pandemic Response Box; Schistosoma mansoni; schistosomiasis
Funding
- Swiss National Science Foundation [320030_175585]
- Swiss National Science Foundation (SNF) [320030_175585] Funding Source: Swiss National Science Foundation (SNF)
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This study identified a new compound MMV1581558 with activity against schistosomula, showing potential for further development as an antischistosomal drug.
Background Treatment and control of schistosomiasis rely on a single drug, praziquantel. New orally active antischistosomals featuring novel molecular scaffolds are urgently needed to prevent the emergence of resistance. Methods We screened 400 drug-like compounds contained in the open-access Pandemic Response Box (PRB) against newly transformed schistosomula (NTS) at a concentration of 10 mu M scoring death, changes in motility, and morphological alterations. Compounds displaying an activity >= 66% at 72 h underwent testing against adult Schistosoma mansoni in vitro. Fast-acting (>= 66% at 24 h), nontoxic drugs focusing on late-stage and approved drugs were investigated in the patent S. mansoni mouse model. Results We identified 26 hits active against NTS, of which 17 elicited >= 66% activity against adult S. mansoni following 24 h of drug exposure. The highest activity against adult S. mansoni was observed with MMV1581558 (EC50 value of 0.18 +/- 0.01 mu M) and nitazoxanide (0.47 +/- 0.07 mu M). Of the five compounds tested in vivo, MMV1581558 and the approved drug ozanimod reduced average worm burden versus controls by 42 % and 36 %, respectively, after a single oral dose of 200 mg/kg bodyweight in mice harboring a chronic S. mansoni infection. Conclusion MMV1581558 discovered from screening the PRB represents a novel antischistosomal scaffold with high in vitro antischistosomal activity amenable to chemical modification for drug development.
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