4.6 Article

Extracellular IL-37 promotes osteogenic and odontogenic differentiation of human dental pulp stem cells via autophagy

Journal

EXPERIMENTAL CELL RESEARCH
Volume 407, Issue 1, Pages -

Publisher

ELSEVIER INC
DOI: 10.1016/j.yexcr.2021.112780

Keywords

Dental pulp stem cells; IL-37; Osteogenic and odontogenic differentiation; Autophagy

Funding

  1. National Natural Science Foundation of China [81873707, 81900962]
  2. Medical Talent Project of Jiangsu Province [ZDRCA2016086]
  3. Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [2018-87]

Ask authors/readers for more resources

IL-37 enhances the osteogenic and odontogenic differentiation ability of dental pulp stem cells (DPSCs) without affecting their proliferation, and also activates cell autophagy. Activation of autophagy promotes osteogenesis and odontogenesis of DPSCs, while inhibition of autophagy suppresses their differentiation.
The osteogenic and odontogenic differentiation of dental pulp stem cells (DPSCs) contribute to restoration and regeneration of dental tissue. Previous study indicated that interleukin-37 (IL-37) was an anti-inflammatory factor that affected other pro-inflammatory signals. The aim of this study was to explore the effects of IL-37 on the differentiation of DPSCs. DPSCs were cultured in growth medium with different concentrations of IL-37. We selected the optimal concentration for the following experiments by alkaline phosphatase (ALP) activity analysis, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blot. Cell counting kit assay (CCK-8) and 5-Ethyny1-2'-Deoxyuridine (EdU) assay were conducted to assess the effects of IL-37 on the proliferation of DPSCs. ALP activity assay and staining, alizarin red S (ARS) staining, qRT-PCR, Western blot as well as immunofluorescence staining were conducted to assess differentiation ability of DPSCs. Western blot, immunofluorescence staining and transmission electron microscopy (TEM) were utilized to examine cell autophagy. Results showed that IL-37 enhanced the osteogenic and odontogenic differentiation ability of DPSCs with no significant influence on the proliferation of DPSCs. Autophagy in DPSCs was activated by IL-37. Activation of autophagy enhanced osteogenesis and odontogenesis of DPSCs, whereas inhibition of autophagy suppressed DPSCs osteogenic and odontogenic differentiation. In conclusion, IL-37 increased osteogenic and odontogenic differentiation via autophagy.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available