Pyrethroid target-site resistance mutations in populations of the honey bee parasite Varroa destructor (Acari: Varroidae) from Flanders, Belgium

The honey bee ectoparasite Varroa destructor is considered the major threat to apiculture, as untreated colonies of Apis mellifera usually collapse within a few years. In order to control this mite, many beekeepers rely on a limited number of approved synthetic acaricides, including the pyrethroids tau-fluvalinate and flumethrin. Due to the intensive use of these products, resistance is now commonplace in many beekeeping regions across the world. In the present study, the occurrence of amino acid substitutions at residue L925 of the voltage-gate sodium channel-the pyrethroid target site-was studied in Varroa populations collected throughout Flanders, Belgium. Dose-response bioassays supported the involvement of the frequently observed L925V substitution in flumethrin resistance, resulting in a 12.64-fold increase of the LC50 in a Varroa population mostly consisting of homozygous 925 V/V mites. With the presence of L925 substitutions in about four out of 10 screened apiaries, the use of pyrethroid-based varroacides in Flanders, including the recently released PolyVar (R) Yellow, should be carefully considered.

Automated summary

The Varroa destructor mite, considered a major threat to beekeeping, has developed resistance to synthetic acaricides like fluvalinate and flumethrin. A study in Flanders, Belgium found that a common L925V substitution in the mites is associated with resistance to flumethrin, leading to a significant increase in resistance levels. This highlights the importance of careful consideration when using pyrethroid-based varroacides in Flanders.