Mineral Composition, Phenolic Content, and In Vitro Antidiabetic and Antioxidant Properties of Aqueous and Organic Extracts of Haloxylon scoparium Aerial Parts

Haloxylon scoparium is a plant widely used in traditional medicine for the treatment of diabetes. Hence, this study focuses on the mineralogical and chemical composition and evaluation of the antidiabetic and antioxidant activities of the aerial part of this species. The mineralogical analysis was done by inductively coupled plasma atomic emission spectrometry (ICP-AES). The phytochemical study consisted in the preparation of different extracts from the aerial part by aqueous and organic extraction using Soxhlet and cold maceration. Then, phytochemical screening was performed on the plant powder and on the extracts, which is completed by spectrophotometric quantification of total polyphenols, flavonoids, and catechic tannins. The evaluation of antidiabetic activity was done by three enzymes: a-amylase, a-glucosidase, and ss-galactosidase, and that of antioxidant activity was done by five methods: H2O2, DPPH, ABTS, FRAP, and reducing power (RP). Mineralogical analysis revealed the presence of iron, potassium, magnesium, phosphorus, sodium, copper, calcium, strontium, selenium, and zinc. The studied part is rich in alkaloids, flavonoids, catechic tannins, and saponins. The methanolic extract is rich in total polyphenols (161.65 +/- 1.52 Ug EAG/mg E), and the ethyl acetate extract has high levels of catechic tannins (23.69 +/- 0.6 Ug EC/mg E). In addition, the decoctate expresses a high flavonoid content of 306.59 +/- 4.35 Ug EQ/mg E. The in vitro evaluation of the antidiabetic activity showed that the decoctate has a higher inhibitory capacity on a-glucosidase (IC50 = 181.7 +/- 21.15 ug/mL) than acarbose (IC50 = 195 +/- 6.12 ug/mL). The results of the antioxidant activity showed that the methanolic extract and the decoctate present a percentage of hydrogen peroxide (H2O2) scavenging (20.91 +/- 0.27 and 16.21 +/- 0.39%) higher than that of ascorbic acid (14.35 +/- 0.002%). Positive correlations obtained between the total polyphenol content and the antioxidant activity of the extracts were studied. A positive correlation of a-amylase inhibitory activity was also recorded with the antioxidant activity tests.

Automated summary

The study focused on the mineralogical and chemical composition of Haloxylon scoparium, as well as its evaluation of antidiabetic and antioxidant activities, The aerial part of the species is rich in alkaloids, flavonoids, catechic tannins, and saponins. The extracts showed high levels of total polyphenols, flavonoids, and catechic tannins, with the decoctate displaying a higher inhibitory capacity on α-glucosidase compared to acarbose. The antioxidant activity of the methanolic extract and decoctate also outperformed ascorbic acid, with positive correlations observed between total polyphenol content and antioxidant activity.