Journal
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Volume 224, Issue -, Pages -Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2021.113725
Keywords
Bcl-2 protein fluorescence probe; Bcl-2 protein quantification; Carcinoma cell visualization; Cell sorting; Early cancer diagnosis
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Funding
- National Nature Science Foundation of China [21672127, 81874288]
- Shandong Provincial Natural Science Foundation [ZR2019LZL004]
- Fundamental Research Funds of Shandong University [2018JC017]
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BPFP1 is a new tool for Bcl-2 protein quantification, carcinoma cell visualization, and cell sorting. It can fluorescently label carcinoma cells by binding to overexpressed Bcl-2 protein and distinguish carcinoma cells from normal tissue cells based on the different Bcl-2 protein expression levels.
Overexpression of the Bcl-2 protein has emerged as a hallmark of carcinoma cells and can be employed as a biochemical biomarker of these cells. Therefore, some Bcl-2 protein fluorescence probes (BPFPs) were designed for Bcl-2 protein quantification and carcinoma cells labeling. The high Bcl-2 protein binding affinity (K-i < 1 nM) and selectivity (over 50,000-fold Bcl-2 protein selectivity against Mcl-1 protein) of BPFP1 endow it with the ability to detect trace amounts of Bcl-2 protein. After being incubated with a range of concentrations of Bcl-2 protein, BPFP1 exhibited the desired fluorescence properties and its fluorescence intensity is proportional to Bcl-2 protein concentration. Therefore, BPFP1 provides a convenient approach for Bcl-2 protein quantification and we could determine the concentration of Bcl-2 protein based on the BPFP1's fluorescence intensity. Subsequent studies revealed that BPFP1 can fluorescently label carcinoma cells by binding to overexpressed Bcl-2 protein in living cells, and can distinguish carcinoma cells (HL-60 cells and ACHN cells) from normal-tissue cells (HUVECs) according to the different Bcl-2 protein expression levels between carcinoma cells and normal tissue cells. In the present study, BPFP1 represents a new tool for Bcl-2 protein quantification, carcinoma cell visualization and cell sorting. Moreover, BPFP1 can be used in the future for early cancer diagnosis by detecting carcinoma cells in patient tissues. (C) 2021 Elsevier Masson SAS. All rights reserved.
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