Comparison of non-invasive Staphylococcus aureus sampling methods on lesional skin in patients with atopic dermatitis

There is evidence that Staphylococcus aureus colonisation is linked to severity of atopic dermatitis. As no gold standard for S. aureus sampling on atopic dermatitis skin lesions exists, this study compared three commonly used methods. In addition, effectiveness of standard skin disinfection to remove S. aureus colonisation from these inflamed skin lesions was investigated. In 30 atopic dermatitis patients, three different S. aureus sampling methods, i.e. detergent scrubbing, moist swabbing and tape stripping, were performed on naive and disinfected skin lesions. Two different S. aureus selective media, mannitol salt agar and chromID agar, were used for bacterial growing. Quantifying the S. aureus load varied significantly between the different sampling methods on naive skin lesions ranging from mean 51 to 1.5 x 10(4) CFU/cm(2) (p < 0.001). The qualitative detection on naive skin was highest with the two detergent-based techniques (86% each), while for tape stripping, this value was 67% (all on chromID agar). In comparison, mannitol salt agar was less sensitive (p < 0.001). The disinfection of the skin lesions led to a significant reduction of the S. aureus load (p < 0.05) but no complete eradication in the case of previously positive swab. The obtained data highlight the importance of the selected sampling method and consecutive S. aureus selection agar plates to implement further clinical studies for the effectiveness of topical anti-staphylococcal antibiotics. Other disinfection regimes should be considered in atopic dermatitis patients when complete de-colonisation of certain skin areas is required, e.g. for surgical procedures.

Automated summary

This study compared three commonly used methods for sampling Staphylococcus aureus on atopic dermatitis skin lesions and investigated the effectiveness of skin disinfection. The results showed significant differences in quantifying S. aureus load between different sampling methods, highlighting the importance of method selection. Skin disinfection led to a reduction in S. aureus load, but complete eradication was not achieved. This data suggests the need for further clinical studies on the effectiveness of topical anti-staphylococcal antibiotics and consideration of alternative disinfection regimes in atopic dermatitis patients.