Journal
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Volume 51, Issue 2, Pages 119-133Publisher
SPRINGER
DOI: 10.1007/s00249-021-01587-5
Keywords
Dynamic force spectroscopy; Micropipette; Cortical tension; Mechanobiology
Categories
Funding
- Australian Research Council (ARC) [DP200101970]
- National Health and Medical Research Council (NHMRC) of Australia [APP2003904]
- NSW Cardiovascular Capacity Building Program [GC-2022]
- Sydney Research Accelerator prize
- NSW CVRN-VCCRI Research Innovation Grant
- Ramaciotti Foundations Health Investment Grant [2020HIG76]
- ARC [DE190100609]
- National Heart Foundation Future Leader Level 2 [FLF2 105863]
- CAUL
- Australian Research Council [DE190100609] Funding Source: Australian Research Council
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Mechanobiology is an emerging field that investigates the roles of mechanical forces in biology. Recent advances in experimental methods allow for measurement of force-dependent interactions and mechanical properties of cells and biomolecules, providing unprecedented insights into mechanobiological processes.
Mechanobiology is an emerging field at the interface of biology and mechanics, investigating the roles of mechanical forces within biomolecules, organelles, cells, and tissues. As a highlight, the recent advances of micropipette-based aspiration assays and dynamic force spectroscopies such as biomembrane force probe (BFP) provide unprecedented mechanobiological insights with excellent live-cell compatibility. In their classic applications, these assays measure force-dependent ligand-receptor-binding kinetics, protein conformational changes, and cellular mechanical properties such as cortical tension and stiffness. In recent years, when combined with advanced microscopies in high spatial and temporal resolutions, these biomechanical nanotools enable characterization of receptor-mediated cell mechanosensing and subsequent organelle behaviors at single-cellular and molecular level. In this review, we summarize the latest developments of these assays for live-cell mechanobiology studies. We also provide perspectives on their future upgrades with multimodal integration and high-throughput capability.
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