Aflatoxin M1 in distributed milks in northwestern Iran: occurrence, seasonal variation, and risk assessment

Aflatoxin is one of the dangerous fungal toxins that is produced in the presence of humidity and heat and lack of proper storage conditions and is considered as a dangerous substance threatening human health. The work aimed to determination of aflatoxin M1 (AFM) level in raw, pasteurized, and sterilized milks offered in the study area and to evaluate the risk of its consumption. In this descriptive cross-sectional study, 60 samples of milk types supplied in the region were collected during two series of sampling (cold and hot seasons) and analyzed by ELISA method. LCR, MoE, and HI indices were used to assess the possible risk of consuming milk containing AFM. In all taken samples AFM was found, the toxin concentration range in the first and second series of sampling was 57.5-270.6 ng/L and 57-185.9 ng/L, respectively. The level of toxin in pasteurized and sterilized milks in both sampling series was higher than raw milks. Based on the obtained data and in order to assess the risk of milk consumption in the target population, associated values with EDI, LCR, MoE, and HI were also calculated and obtained in the range of 0.145-0.3 ng/k.d b.w, 0.0008-0.0017 additional case per one million population, 1892.9-3921.6 and 0.72-1.5 ng/kg b.w, respectively. According to the findings, all samples tested are contaminated and although it is within the range of the WHO recommendation, however, based on the calculated indicators, the risk of liver cancer threatens the consumers of these milks. Therefore, it is necessary to manage this issue through educational control and monitoring measures.

Automated summary

This study investigated the level of aflatoxin M1 (AFM) in different types of milk in a specific area and assessed the risk of consuming these milks. Results showed that all samples tested were contaminated and had the potential to pose a risk of liver cancer to consumers, indicating the need for educational control and monitoring measures.