Genotoxicity assessment in two Amazonian estuaries using the Plagioscion squamosissimus as a biomonitor

Genotoxicity studies in coastal ecosystems have been a priority in Environmental Risk Assessment (ERA). This research aimed to study the genotoxicity by the micronucleus test and comet assay in two Brazilian Amazon estuaries (anthropized and control) using Plagioscion squamosissimus as a biomonitor. Blood samples were collected from 54 specimens. No significant genotoxic effects were detected in the cells analyzed, although the highest occurrence (MN and DNA damages) was observed in anthropized site. The percentage of genomic damage differed between the sites studied, being always higher in anthropizes site as well. Of the nucleoids analyzed in this site, on average, 28 +/- 14.42% of the cells were classified in the highest damage class. The fish analyzed in the present study are direct influenced of xenobiont agents capable of producing damage to the genetic material of aquatic organisms in both sites and, consequently, may bring consequences still little reported in studies of morphophysiological alterations in humans.

Automated summary

Genotoxicity studies on Plagioscion squamosissimus in two different Brazilian Amazon estuaries revealed no significant effects, but the anthropized site showed higher occurrence of DNA damages. The fish in these sites are influenced by xenobiont agents which can cause genetic material damage in aquatic organisms, potentially leading to morphophysiological alterations in humans.