Transcriptomics and transmission ultrastructural examination reveals the nephrotoxicity of cadmium in laying hens

The objective of this study was to reveal the effects of cadmium (Cd) on ultrastructural changes, oxidative stress, and transcriptome expression in the kidneys of laying hens. Seventy-two healthy Hy-Line brown laying hens at 41 weeks old were randomly allocated to four treatment groups with six replicates. The control group received a basal diet without additional Cd incorporation, and the other three treatment groups received diets supplemented with 15, 30, or 60 mg Cd /kg of feed. After 6 weeks of exposure, the results show that administration of 60 mg/kg Cd significantly reduced (P < 0.05) eggshell thickness. With an increase in the Cd concentration in feed, the concentrations of renal Zn and Fe also had changed. Renal histopathology and ultrastructure also showed aggravated damage to glomeruli and renal tubules and the deformation of nuclei and mitochondria in all Cd treatment groups. With an increase in Cd in feed, the activity of glutathione peroxide (GPX) and catalase (CAT) was significantly reduced (P < 0.05), while the activity of total antioxidant capacity (T -AOC) was decreased (P < 0.05) only in the 60 mg/kg Cd group. RNA-seq analysis revealed that 410 genes displayed differential expression (>= 1.5-fold) in the 60 mg/kg supplementation group, compared to the control group. GO and KEGG pathway analysis results showed that Cd affected many genes involved in mitochondria and ion transport. In conclusion, this study elaborates the mechanisms underlying renal toxicity caused by Cd, which might provide target candidate genes for alleviating Cd poisoning in laying hens.

Automated summary

This study investigated the effects of cadmium on kidney ultrastructural changes, oxidative stress, and transcriptome expression in laying hens. Results showed that high levels of dietary cadmium caused thinning of eggshells, increased renal damage, and reduced antioxidant capacity. RNA-seq analysis revealed differential expression of genes related to mitochondria and ion transport. These findings provide insights into the mechanisms of cadmium toxicity in laying hen kidneys and offer potential candidate genes for mitigating cadmium poisoning.