Inoculation of Azospirillum brasilense and exogenous application of trans-zeatin riboside alleviates arsenic induced physiological damages in wheat (Triticum aestivum)

Due to increased industrialization, arsenic (As) in the soil has become a serious issue for wheat production since past few decades. We investigated the role of Azospirillum brasilense and trans-zeatin riboside (tZR) in the mitigation of arsenic toxicity in wheat for 2 years (2018-2019 and 2019-2020) in pot experiments. Wheat plants grown in soil artificially spiked with arsenic (50, 70, and 100 mu M) was left alone or amended with A. brasilense, tZR, or their combination as mitigation strategies. A treatment without arsenic or amendments was maintained as control. Arsenic-induced physiological damages were noticed in the wheat plants. Detrimental effects on the plant physiological functions, such as disruption of cell membrane stability, reduced water uptake, and stomatal functions, were noticed with increase in As toxicity. Application of biological amendments reversed the effects of As toxicity by increasing wheat plant growth rate, leaf area, and photosynthesis and also yield. Therefore, application of tZR and wheat seed inoculation with A. brasilense could be a sustainable and environmentally friendly strategy to mitigate arsenic-induced crop physiological damages.

Automated summary

This study investigated the role of Azospirillum brasilense and trans-zeatin riboside (tZR) in mitigating arsenic toxicity in wheat. The results showed that the application of these biological amendments reversed the physiological damages caused by arsenic toxicity and increased wheat growth and yield.