Comparison of the toxicity of pure and samarium-doped zinc oxide nanoparticles to the green microalga Chlorella vulgaris

Although doping of various rare earth elements such as samarium on zinc oxide nanoparticles (ZnO NPs) can noticeably improve their photocatalytic performance, it may enhance their toxicity to living organisms. Thus, the toxic impacts of samarium-doped ZnO NPs (Sm/ZnO NPs) on different organisms should be carefully evaluated. In this study, an eco-toxicological experimentation system using the green microalga Chlorella vulgaris was established to determine the potential toxicity of ZnO and Sm/ZnO NPs synthesized by polymer pyrolysis method. Accordingly, growth parameters, oxidative stress biomarkers, and morphological features of the algal cells were analyzed. Both ZnO and Sm/ZnO NPs induced a concentration-dependent cytotoxicity by reducing the cell growth, decreasing photosynthetic pigment contents, and causing deformation in the cellular morphology. Moreover, generation of excessive H2O2, increased activity of superoxide dismutase and ascorbate peroxidase, and reduction in total phenolic and flavonoid contents were observed. Catalase activity was inversely influenced by the NPs in a way that its activity significantly increased at the concentrations of 20 and 25 mg L-1 of ZnO NPs, but was lessened by all supplemented dosages (5-25 mg L-1) of Sm/ZnO NPs. Altogether, the obtained results revealed that Sm-doping can play a significant role in ZnO NP-induced toxicity on C. vulgaris cells.

Automated summary

This study investigates the potential toxicity of zinc oxide nanoparticles (ZnO NPs) and samarium-doped ZnO NPs (Sm/ZnO NPs) on the green microalga Chlorella vulgaris. The results show that both ZnO and Sm/ZnO NPs induce concentration-dependent cytotoxicity, including reduced cell growth, decreased photosynthetic pigment contents, and deformation in the cellular morphology. Sm-doping plays a significant role in enhancing the toxicity of ZnO NPs on C. vulgaris cells.