Increase of antibiotic resistance genes via horizontal transfer in single- and two-chamber microbial electrolysis cells

Microbial electrolysis cells (MECs) have been applied for antibiotic degradation but simultaneously induced antibiotic resistance genes (ARGs), thus representing a risk to disseminate antibiotic resistance. However, few studies were on the potential and risk of ARGs transmission in the MECs. This work assessed conjugative transfer of ARGs under three tested conditions (voltages, cell concentration, and donor/recipient ratio) in both single- and two-chamber MECs. The results indicated that voltages (> 0.9 V) facilitated the horizontal frequency of ARGs in the single-chamber MECs and anode chamber of two-chamber MECs. The donor cell number (donor/recipient ratio was 2:1) increased the transfer frequency of ARGs. Furthermore, voltages ranged from 0.9 to 2.5 V increased reactive oxygen species (ROS) production and cell membrane permeability in MECs. These findings offer new insights into the roles of ARG transfer under different applied voltages in the MECs, which should not be ignored for horizontal transfer of antibiotic resistance.

Automated summary

This study assessed the risk of antibiotic resistance gene (ARGs) transmission in microbial electrolysis cells (MECs) and explored the mechanisms of ARG transfer under different voltage conditions. The results showed that voltage and donor cell number significantly influenced the frequency of ARG transfer, and high voltage increased the production of reactive oxygen species and cell membrane permeability.