Sodium hydrosulfite together with silicon detoxifies arsenic toxicity in tomato plants by modulating the AsA-GSH cycle

The main intent of the current research was to appraise if combined application of hydrogen sulfide (H2S, 0.2 mM) and silicon (Si 2.0 mM) could improve tolerance of tomato plants to arsenic (As as sodium hydrogen arsenate heptahydrate, 0.2 mM) stress. Plant growth, chlorophylls (Chl), PSII maximum efficiency (Fv/Fm), H2S concentration and L-cysteine desulfhydrase activity were found to be suppressed, but leaf and root As, leaf proline content, phytochelatins, malondialdehyde (MDA) and H2O2 as well as the activity of lipoxygenase (LOX) increased under As stress. H2S and Si supplied together or alone enhanced the concentrations of key antioxidant biomolecules such as ascorbic acid, and reduced glutathione and the activities of key antioxidant system enzymes including catalase (CAT), superoxide dismutase (SOD), dehydroascorbate reductase (DHAR), glutathione reductase (GR), and glutathione S-transferase (GST). In comparison with individual application of H2S or Si, the joint supplementation of both had better effect in improving growth and key biochemical processes, and reducing tissue As content, suggesting a putative collaborative role of both molecules in improving tolerance to As-toxicity in tomato plants.

Automated summary

The study aimed to evaluate the combined application of hydrogen sulfide and silicon on improving tolerance of tomato plants to arsenic stress. The results showed that arsenic stress inhibited plant growth and key biochemical processes, while the joint supplementation of hydrogen sulfide and silicon enhanced antioxidant biomolecules and reduced arsenic content, indicating a collaborative role of both molecules in improving tolerance to arsenic toxicity in tomato plants.