Journal
ELECTROANALYSIS
Volume 34, Issue 3, Pages 535-541Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202100436
Keywords
point-of-care testing (POCT); aptasensor; nanozyme; pH-metric biosensor; nanoceria
Categories
Funding
- National Natural Science Foundation of China [21874129, 22174137]
- International Scientific Cooperation Project of Jilin Scientific and Technological Development Program [20200801044GH]
- Key Research and Development Projects of Jilin Scientific and Technological Development Program [202102041266YY]
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In this study, an ultrasensitive pH-metric sandwich-type aptasensor was developed using CeO2 nanorods and MFP for detecting thrombin, showing eligible selectivity and detection limit of 1.17 nM.
The demand for point-of-care testing (POCT) is growing dramatically, especially for district where health facilities are poorly staffed, poorly skilled and ill-equipped. As a commercialized portable device, pH meters can be used for detection of various targets, relying on bioactive enzymes. The nanozymes, as the alternative of the natural enzymes, have rarely been used for pH-metric POCT strategies. Herein, we developed an ultrasensitive pH-metric sandwich-type aptasensor based on the CeO2 nanorods (CeO2 NRs) as phosphatase-mimic and sodium monofluorophosphate (MFP) as catalytic substrate. Under optimal conditions, such strategy yields a detection limit of 1.17 nM with eligible selectivity for detecting thrombin.
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