Journal
ELECTROANALYSIS
Volume 34, Issue 7, Pages 1187-1192Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202100560
Keywords
Streptococcus uberis; mastitis; mammary epithelial cells; pyruvate; nicotinamide adenine dinucleotide; differential pulse voltammetry
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Funding
- Natural Science Foundation of Jiangsu Province for Excellent Young Scholars [BK20170087]
- Independent Innovation of Agricultural Science and Technology in Jiangsu Province of China [CX(21)3122]
- Sanya Nanjing Agricultural University Research Institute guiding fund project [NAUSYMS11]
- Program for Student Innovation [1917C06]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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A novel electrochemical immunosensor for lactate dehydrogenase (LDH) detection was developed, which demonstrated high sensitivity and potential application in disease diagnosis.
A novel electrochemical immunosensor for lactate dehydrogenase (LDH) detection was proposed based on analyte-driven catalytic reaction by attaching LDH antibodies on multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) modified glassy carbon electrodes (GCE). As LDH was captured by the antibodies on electrode surface, it catalyzed the formation of pyruvate and the reduced form of nicotinamide adenine dinucleotide (NADH), thus a sensitive electrochemical signal obtained from the above redox reaction was recorded by differential pulse voltammetry (DPV). Under optimum conditions, the developed immunosensor exhibits high sensitivity for LDH quantification ranging from 0.001 mu g/mL to 0.5 mu g/mL with a low detection limit at 0.39 ng/mL. This developed immunosensor reveals ideal accuracy and feasibility for LDH detection in Streptococcus uberis (S. uberis) induced bovine mammary epithelial cells (MECs) samples by comparison with conventional commercial kit, which shows remarkably application potential in diseases diagnosis.
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