Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 225, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2021.112730
Keywords
Vitamin D; Silica; Macrophage autophagy; Pulmonary inflammatory injury
Categories
Funding
- National Natural Science Foundation of China [81703205]
- Priority Academic Program Development of Jiangsu Higher Educa-tion Institutions (PAPD)
- Foundation from Chongqing Yuzhong District Science and Technology Bureau [201930]
- Natural Science Foundation of Chongqing [cstc2020jcyj-msxm3187]
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The study identifies the harmful effect of crystalline silica on lung tissue and the protective mechanism of vitamin D involving M2 macrophages autophagy that mitigates crystalline silica-induced lung injury.
Crystalline silica (CS) is a universal environmental pollutant, which causes a typical inflammatory lung injury. Vitamin D shows huge potential against particles-induced lung injury, while little known about the molecular mechanism involved in macrophage autophagy. In this study, we aim to identify the protective effects of vitamin D on CS caused lung inflammatory injury and clarify the detail mechanism. After exposure to CS (3 mg/mice in 50 mu l PBS), wildtype and Atg7(flox/flox) Lyz2-cre mice were treated with or without vitamin D-3 (40,000 IU/kg). The results indicated that exposure to CS caused an obvious lung injury, manifesting as pathological structural changes, macrophage-dominated inflammatory cell infiltration and increased pro-inflammatory cytokines. Remarkably, these damages were more serious in Atg7(flox/flox) Lyz2-cre mice. Vitamin D was found to inverse CS-induced inflammatory cell infiltration and restored anti-inflammatory M2 macrophages by inducing autophagy, which attenuated lung injury, as determined by decreased levels of apoptosis and inflammatory response. While, this effects of vitamin D were slashed in Atg7(flox/flox) Lyz2-cre mice. This study reveals the adverse effect of CS on lung tissue and the protective mechanism of vitamin D involved in M2 macrophages autophagy, which attenuates CS-caused lung injury.
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