Journal
DYES AND PIGMENTS
Volume 194, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.109636
Keywords
Berberrubine; Fluorescent probe; Ratiometric; pH sensor; Diabetic ketoacidosis
Funding
- CSIR
- UGC
Ask authors/readers for more resources
Berberrubine (BBn) is a ratiometric naturally occurring fluorescent pH probe for the direct detection of diabetic ketoacidosis (DKA) conditions in patients with type I diabetes mellitus. The probe exhibits remarkable changes in absorption spectra and fluorescence enhancement under acidic conditions, showing good linearity with pH variations and potential applications in acidic microenvironments.
Herein we report a ratiometric naturally occurring fluorescent pH probe, berberrubine (BBn) for the direct detection of diabetic ketoacidosis (DKA) conditions of patients having type I diabetes mellitus. The photophysical properties of the probe during pH titrations showed remarkable changes in absorption spectra where two absorption bands at 377 and 326 nm have disappeared followed by the emergence of an absorption maxima at 346 nm in highly acidic conditions. In addition, a fluorescence enhancement effect was observed in the alkaline pH, with a bathochromic shift of 33 nm. Moreover, the solution switches the color from light yellow to light pink with the change of pH from acidic to basic. A pKa value of 7.57 and a good linearity between pH 5.0-9.0 indicate that the probe can be used efficiently for the DKA condition, where pH variations are in the range of 6-7. The excellent water solubility, photostability, reversibility, and selectivity of BBn make it a potential pH sensing agent for acidic microenvironments. The reversible sensing of pH variations during DKA could be effective in primary detection and diagnosis which can assist in avoiding further complications of acidosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available