4.3 Article

Accuracy of ASTA MicroIDSys, a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system, for the identification of Korean reference and clinical bacterial and yeast strains

Journal

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2022.115658

Keywords

MALDI-TOF mass spectrometry; ASTA MicroIDSys system; Bruker biotyper system; Identification; Bacteria; Yeast

Funding

  1. Chosun University Hospital [2020-28]

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This study compared the accuracy of the ASTA MicroIDSys system and the MALDI Biotyper system in identifying bacterial and yeast strains, and found that the MicroIDSys system showed high accuracy and utility in identifying bacteria and yeast.
This study compared the accuracy of a new MALDI-TOF mass spectrometry system, ASTA MicroIDSys system, with that of MALDI Biotyper system for the identification of reference and clinical bacterial and yeast strains. The identification accuracy of the 2 systems was compared using a total of 406 strains comprising 142 aerobic and 180 anaerobic bacterial strains and 84 yeast strains. The genus and species identification rates were 98.0% and 89.4% using MicroIDSys and 96.1% and 89.4% using Biotyper, respectively. The species identification rates of MicroIDSys and Biotyper for aerobic bacteria were 93.0% and 97.2%, respectively, and those for anaerobic bacteria were 85.6% and 81.7%, respectively. The accuracy of yeast identification at the species level was 91.7% using MicroIDSys and 92.9% using Biotyper. These findings indicate that MicroIDSys could be useful for the accurate identification of bacteria and yeast in clinical microbiology laboratories. (c) 2022 Published by Elsevier Inc.

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