4.4 Article

Mechano-chemical feedback mediated competition for BMP signalling leads to pattern formation

Journal

DEVELOPMENTAL BIOLOGY
Volume 481, Issue -, Pages 43-51

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2021.09.006

Keywords

Drosophila melanogaster; epithelial cells; apical constriction; BMP signalling; Cell competition

Funding

  1. Institute of Biotechnology of the University of Helsinki
  2. Academy of Finland [308045, 295013]
  3. Sigrid Juselius Foundation
  4. Center of Excellence in Experimental and Computational Developmental Biology from the Academy of Finland
  5. Academy of Finland (AKA) [295013, 295013, 308045, 308045] Funding Source: Academy of Finland (AKA)

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Developmental patterning is regulated by conserved signaling pathways, with cell shape changes playing a critical role in pattern refinement. Mechano-chemical feedback leads to competition for developmental signals, affecting the process of pattern refinement.
Developmental patterning is thought to be regulated by conserved signalling pathways. Initial patterns are often broad before refining to only those cells that commit to a particular fate. However, the mechanisms by which pattern refinement takes place remain to be addressed. Using the posterior crossvein (PCV) of the Drosophila pupal wing as a model, into which bone morphogenetic protein (BMP) ligand is extracellularly transported to instruct vein patterning, we investigate how pattern refinement is regulated. We found that BMP signalling induces apical enrichment of Myosin II in developing crossvein cells to regulate apical constriction. Live imaging of cellular behaviour indicates that changes in cell shape are dynamic and transient, only being maintained in those cells that retain vein fate competence after refinement. Disrupting cell shape changes throughout the PCV inhibits pattern refinement. In contrast, disrupting cell shape in only a subset of vein cells can result in a loss of BMP signalling. We propose that mechano-chemical feedback leads to competition for the developmental signal which plays a critical role in pattern refinement.

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