Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 124, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2021.104185
Keywords
circRNAs; Megalobrama amblycephala; Aeromonas hydrophila; Immune response; ceRNA network; WGCNA
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Funding
- National Natural Science Foundation of China [31972781]
- Earmarked Fund for China Agriculture Research System titled as-Staple Freshwater Fishes Industry Technology System [CARS4608]
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In this study, 250 circRNAs were identified in Megalobrama amblycephala liver post Aeromonas hydrophila infection, with GO and KEGG analyses revealing enrichment of DE circRNAs parental genes in phagocytosis, complement and coagulation cascades, and Fc gamma R-mediated phagocytosis pathways. The ceRNA interaction network and WGCNA analysis further highlighted the important role of circRNAs in immune response, providing valuable insights into the immune response mechanism of M. amblycephala to A. hydrophila.
Circular RNAs (circRNAs), a class of non-coding RNAs, play an important role in regulating various biological processes. In the present study, circRNAs from the Megalobrama amblycephala liver were identified at five different time points post Aeromonas hydrophila using RNA-seq technology. A total of 250 circRNAs were identified, of which 106 were differentially expressed (DE) in ten pairwise comparisons. GO and KEGG analyses showed that the parental genes of DE circRNAs were enriched in phagocytosis, complement and coagulation cascades, and Fc gamma R-mediated phagocytosis pathways. According to ceRNA hypothesis, the interaction network of circRNAs, miRNAs and mRNAs was constructed. Moreover, WGCNA was conducted, and five specific modules significantly related to bacterial infection were identified. All the above results reveal the important role of circRNAs in immune response, which enriches the information of circRNAs in teleost, and helps to understand the immune response mechanism of M. amblycephala to A. hydrophila.
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