Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 125, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2021.104174
Keywords
Arasin-like; Immune response; Litopenaeus vannamei; Antibacterial activity; RNA interference
Categories
Funding
- National Key R&D Program of China [2019YFD0900200, 2018YFD0900500, 2018YFD0900600]
- National Natural Science Foundation of China [32072988, 32022085]
- General Program of Natural Science Foundation of Guangdong Province, China [2020A1515010319, 2018A030313963]
- Key-Area Research and Development Program of Guangdong Province [2018B020204001]
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The LvArasin-like peptide is identified from the hemocytes of Pacific white shrimp and exhibits antimicrobial activity against both gram-positive and gram-negative bacteria. Its expression is up-regulated in response to bacterial infections, suggesting its involvement in host defense. LvArasin-like has the potential to be a therapeutic agent for disease control in shrimp aquaculture.
Antimicrobial peptides (AMPs) play an important role in the host defense system of shrimps. In this study, an Arasin-like peptide, named as LvArasin-like, was identified from the hemocytes of the pacific white shrimp, Litopenaeus vannamei. The complete open reading frame (ORF) of LvArasin-like was 213 bp, encoding 70 amino acid residues with a predicted molecular mass of 5.68 kDa and a theoretical isoelectric point (pI) of 6.73. The predicted peptide consisted of a signal peptide, an N-terminal Pro/Arg-rich domain, and a C-terminal cysteinerich domain. LvArasin-like expression was most abundant in the gills and was up-regulated in hemocytes after LPS or Poly I:C injection as well as challenges by Vibrio parahaemolyticus or Staphylococcus aureus infection. In the hetemlogous expression system, LvArasin-like protein (rLvArasin-like) was recombinantly expressed in the forms of a dimer or both a monomer and dimer. The rLvArasin-like could directly bind to gram-positive and gramnegative bacteria and exhibited broad-spectrum antimicrobial activity towards them, with 50 % of minimal inhibitory concentrations (MIC50) of 6.25-50 mu M. Moreover, dsRNA-mediated knockdown of LvArasin-like enhanced the susceptibility of shrimp to V. parahaemolyticus. In addition, the transcriptional level of LvArasin-like was downregulated when silencing of the transcription factors LvDorsal and LvRelish using RNAi in vivo. All of these results suggest that LvArasin-like is involved in host defense against bacterial infection. Therefore, it is a potential therapeutic agent for disease control in shrimp aquaculture.
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