Journal
DEVELOPMENT
Volume 148, Issue 24, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.199675
Keywords
Selfing; Isogenic; Forward genetics; Tail bud; Noto; Mesogenin; Mutant; RNA-seq
Categories
Funding
- University of Basrah, Iraq
- National Institute of Child Health and Human Development [R15HD060017]
- Valdosta State University Faculty Seed Research Grant [FRSG-FY2012]
- Biotechnology and Biological Sciences Research Council [BB/V000411/1, BB/P025528/1]
- University of Exeter
Ask authors/readers for more resources
This study characterized two mutants in self-fertilizing mangrove killifish, with identified mutated genes for rapid identification. The findings showcase the potential for accelerating future small-scale forward-genetic screening and mutation identification.
Using the self-fertilizing mangrove killifish, we characterized two mutants, shorttail (stl) and balltail (btl). These mutants showed abnormalities in the posterior notochord and muscle development. Taking advantage of a highly inbred isogenic strain of the species, we rapidly identified the mutated genes, noto and msgn1 in the stl and btl mutants, respectively, using a single lane of RNA sequencing without the need of a reference genome or genetic mapping techniques. Next, we confirmed a conserved morphant phenotype in medaka and demonstrate a crucial role of noto and msgn1 in cell sorting between the axial and paraxial part of the tail mesoderm. This novel systemcould substantially accelerate future small-scale forward-genetic screening and identification of mutations. Therefore, the mangrove killifish could be used as a complementary system alongside existing models for future molecular genetic studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available