Intelligent pH-responsive dental sealants to prevent long-term microleakage

Objectives. Microleakage is a determinant factor of failures in sealant application. In this study, DMAEM (dodecylmethylaminoethyl methacrylate), a pH-responsive antibacterial agent, was incorporated into resin-based sealant for the first time. The objectives of this study were to: (1) investigate the long-term performance of DMAEM-modified sealants against oral microbial-aging; and (2) investigate the long-term preventive effect of DMAEMmodified sealants on microleakage. Methods. Depth-of-cure and cytotoxicity of DMAEM-modified sealants were measured. Then, an aging model using biofilm derived from the saliva of high caries experience children was conducted. After aging, microhardness and surface roughness were measured. Biofilm activity, lactic acid production and exopolysaccharide (EPS) production were measured. 16S rRNA gene sequencing were also performed. The effects of DMAEM on microleakage were tested using an in vitro microleakage assessment. Results. The addition of DMAEM with a mass fraction of 2.5-10% did not affect depth-ofcure values and cytotoxicity of sealants. Adding 2.5-10% DMAEM did not affect the surface roughness and microhardness after aging. Compared to control, adding 2.5-10% DMAEM reduced biofilm metabolic activity by more than 80%. The lactic acid production and EPS production were reduced by 50% in DMAEM groups. DMAEM-modified sealants maintained the microbial diversity of biofilm after aging, they also inhibited the growth of lactobacillus. The 5% and 10% DMAEM groups exhibited a significant reduction in microleakage compared to control. Significance. The long-term antibacterial activities against oral microbial-aging and the longterm microecosystem-regulating capabilities enabled DMAEM-modified sealant to prevent microleakage in sealant application and thus prevent dental caries. (c) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.

Automated summary

The DMAEM-modified sealants showed promising long-term antibacterial activities against oral microbial-aging and long-term microecosystem-regulating capabilities, resulting in a significant reduction in microleakage and potential prevention of dental caries without affecting the depth-of-cure values and cytotoxicity of the sealants.