Comprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions

The B cell compartment provides innate and adaptive immune defenses against pathogens. Different B cell subsets, reflecting the maturation stages of B cells, have noninterchangeable functions and roles in innate and adaptive immune responses. In this review, we provide an overview of the B cell subsets present in peripheral blood of healthy individuals. A specific gating strategy is also described to clearly and univocally identify B cell subsets based on the their phenotypic traits by flow cytometric analysis.

Automated summary

The B cell compartment consists of different subsets with unique functions and roles in immune responses. A specific gating strategy is described for identifying these subsets based on phenotypic traits using flow cytometric analysis.