Microbial communities and their enzymes facilitate degradation of recalcitrant polymers in anaerobic digestion

Microbial consortia efficiently degrade complex biopolymers found in the organic fraction of municipal solid waste (OFMSW). Through enzyme production and division of labor during anaerobic digestion, microbial communities break down recalcitrant polymers and make fermentation products, including methane. However, microbial communities remain underutilized for waste degradation as it remains difficult to characterize and predict microbial interactions during waste breakdown, especially as cultivation conditions change drastically throughout anaerobic digestion. This review discusses recent progress and opportunities in cultivating natural and engineered consortia for OFMSW hydrolysis, including how recalcitrant substrates are degraded by enzymes as well as the critical factors that govern microbial interactions and culture stability. Methods to measure substrate degradation are also reviewed, and we demonstrate the need for increased standardization to enable comparisons across different environments.

Automated summary

This review discusses the recent progress and opportunities in cultivating natural and engineered microbial consortia for organic fraction of municipal solid waste (OFMSW) hydrolysis. It emphasizes the degradation of recalcitrant substrates by enzymes and the critical factors governing microbial interactions and culture stability. The need for increased standardization in measuring substrate degradation is also highlighted to enable comparisons across different environments.