Dissecting intercellular and intracellular signaling networks with barcoded genetic tools

The power of next-generation sequencing has stimulated the development of many analysis techniques for transcriptomics and genomics. More recently, the concept of 'molecular barcoding' has broadened the spectrum of sequencing-based applications to dissect different aspects of intracellular and intercellular signaling. In these assay formats, barcode reporters replace standard reporter genes. The virtually infinitive number of expressed barcode sequences allows high levels of multiplexing, hence accelerating experimental progress. Furthermore, reporter barcodes are used to quantitatively monitor a variety of biological events in living cells which has already provided much insight into complex cellular signaling and will further increase our knowledge in the future.

Automated summary

Next-generation sequencing has led to the development of many analysis techniques for transcriptomics and genomics, while the concept of 'molecular barcoding' has further expanded the sequencing-based applications. Reporter barcodes allow for quantitative monitoring of biological events, providing insights into complex cellular signaling.